软枣猕猴桃多糖的分离纯化及抗氧化活性测定

对微波辅助提取的软枣猕猴桃多糖进行分离纯化并对各纯化组分的抗氧化活性进行测定。利用DE-AE-纤维素阴离子交换层析对软枣猕猴桃多糖进行初步分离,得到1个水洗组分和3个盐洗组分;利用Sephade-xG-100、G-200凝聚柱层析对其进行进一步分离纯化。结果表明:4个组分都为均一多糖且都不含有蛋白质;软枣猕猴桃多糖对DPPH自由基和羟基自由基具有一定的清除能力,对超氧阴离子自由基的清除能力很弱,盐洗组分的抗氧化活性明显优于水洗组分;0.1盐洗组分(0.1 mol/L NaCl溶液洗脱的组分)、0.2盐洗组分(0.2 mol/L NaCl溶液洗脱的组分)、0.3盐洗组分(0.3 mol/L NaCl溶液洗脱的组分)、Vc清除DPPH自由基的IC50分别为0.57、1.61、1.18、0.03 mg/mL;清除羟基自由基的IC50分别为1.5、5.6、2.7、0.2 mg/mL;0.1盐洗组分为软枣猕猴桃多糖中主要的抗氧化活性组分。

Purification and antioxidant activity of polysaccharide from Actinidia arguta

Actinidia arguta polysaccharide extracted by microwave was purified and the antioxidant activities of different fractions were measured.One water elution fraction and three brine elution fractions were separated using a DEAE anion-exchange column,SephadexG-100.SephadexG-200 column were used for further purification.The results indicated the four fractions were homogeneous and did not contain protein.The results of antioxidant activity indicated Actinidia arguta polysaccharide had definite scavenging effects on DPPH· and ·OH,and the scavenging effects on O-2·were indistinctive;antioxidant activity of brine elution fractions were obviously stronger than water elution fraction;the IC50 scavenging DPPH· and ·OH of 0.1 NaCl elution fraction,0.2 NaCl elution fraction,0.3 NaCl elution fraction and Vc were 0.57 mg/mL,1.61 mg/mL,1.18 mg/mL,0.03 mg/mL and 1.5 mg/mL,5.6 mg/mL,2.7 mg/mL,0.2 mg/mL,respectively;0.1 NaCl elution fraction was the main antioxidant activity component in Actinidia arguta polysaccharide.