耐硒驯化灵芝菌种的液态发酵中硒的富集特征 |
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作者单位: | 1. 江南大学生物工程学院,江苏无锡214036 中国科学技术大学苏州研究院,江苏苏州215123 2. 中国科学技术大学苏州研究院,江苏苏州215123 苏州硒谷科技有限公司,江苏苏州215123 3. 中国科学技术大学苏州研究院,江苏苏州,215123 4. 江南大学生物工程学院,江苏无锡,214036 5. 苏州硒谷科技有限公司,江苏苏州,215123 |
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基金项目: | 国家自然科学基金,中国博士后自然科学基金 |
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摘 要: | 对灵芝(Ganoderma lucidum)菌株在平板上做耐硒驯化,然后对驯化前后灵芝菌种液态发酵过程中富硒性能进行了比较,最后对驯化灵芝液态发酵中硒富集特征进行了研究。实验结果表明,灵芝菌种经耐硒驯化后,其生物量、菌丝体硒含量、总硒含量最高分别达到16.1 g/L,13 264μg/L和91.5 mg/L,明显优于未驯化组。对驯化灵芝液态培养过程中硒富集特征研究表明,富硒灵芝中存在硒代蛋氨酸(SeMet)、硒代胱氨酸(SeCys2)、硒甲基硒代半胱氨酸(SeMeCys)和无机硒(Se(IV))等4种硒形态,其中SeMet、SeCys2占主要部分,分别可达硒代氨基酸总量的60%~90%、10%~40%;在培养过程中单一增加Na2SeO3添加浓度,并不能显著增加灵芝中硒代氨基酸含量,而更多以Se(IV)形式存在。
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关 键 词: | 灵芝 富硒 驯化 硒形态 硒代氨基酸 |
Characterization of Selenium Accumulation on Domesticated Ganoderma lucidum in Liquid Fermentation |
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Abstract: | Ganoderma lucidum strain was domesticated in Na2SeO3 medium to investigate its selenium(Se) accumulation ability.Atomic Fluorescence Spectrometry(AFS) and Liquid Chromatographic-Atomic Fluorescence Spectrometry(LC-UV-AFS) were used to determine the total Se contents and Se speciation in cultured Ganoderma lucidum,respectively.The results showed that the largest harvested biomass could reach 16.1 g/L and the respondent Se content could reach 13264 μg/g after the Ganoderma lucidum strain was domesticated by Na2SeO3 medium,which were significantly better than those in non-domesticated ones.SeMet,SeCys2,SeMeCys and inorganic selenium(Se(IV)) could be determined in cultured Ganoderma lucidum,and SeMet and SeCys2 fractions could be 60%~90% and 10%~40%,respectively.It should be pointed out that the Se species was dominated by Se(IV) instead of Se-amino acid if only the contents of Na2SeO3 was increased. |
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Keywords: | Ganoderma lucidum selenium-enriched domestication selenium speciations selenium amino acid |
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