绿僵菌Ma83固态发酵几丁质酶和部分酶学性质的初步研究

研究了金龟子绿僵菌MetarhiziumanisopliaeMa83菌株产几丁质酶的固态发酵条件和粗酶液的酶学性质。研究结果显示 ,当以麸皮∶蚕蛹粉为 3∶1作为培养基 ,最适氮源为1%NaNO3,起始pH为 6 5 ,发酵温度为 31℃ ,接种量为 2mL液态种子时酶活力最高。此外 ,添加稻壳对Ma83产几丁质酶有促进作用。该菌株在生长 2d时 ,酶活力达 33 9U /g干培养基。酶的最适反应温度为 5 0℃ ,最适反应 pH为 6 0 ;不同温度保温 1h后 ,酶的半失活温度为 42℃。不同 pH值的缓冲溶液中 (2 5℃ )放置 1h后 ,酶在pH 5 0～ 6 0条件下稳定性最高

Study on Production Conditions and Properties of Chitinase by Metarhizium anisopliae Ma83

Chitinase production by strain Metarhizium anisopliae Ma83 with solid state fermentation process were studied The result of condition for strain Ma83 chitinase production showed that the wheat bran and silkworm pupa powder at a ratio of 3∶1 as culture medium with an initial pH 6 5, the optimal nitrogen source NaNO 3 concentration at 1?g/L and temperature of 31℃ were the most suitable for chitinase production Additional rice husk also effected on chitinase production Under optimal conditions, maximum chitinase activity reached 33 9?U/g dry medium per gram when cultured for 2 days The properties of chitinase by Ma83 were investigated by solid state fermentation process The optimal reaction pH was 6 0 and temperature was 50℃, respectively The temperature for loss half activity in an hour was 42℃ The stability of the enzyme was tested in a solution of pH 4 0～9 0 at 25℃ for 1?h The chitinase was stable in a range of pH 5 0～6 0.