| 酶法制备汉麻籽蛋白抗氧化肽 |
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| 引用本文： | 周徐慧,钱平,卢蓉蓉,陈天鹏,史玲,何锦风,王璋. 酶法制备汉麻籽蛋白抗氧化肽[J]. 食品与发酵工业, 2008, 34(5): 76-81 |
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| 作者姓名： | 周徐慧 钱平 卢蓉蓉 陈天鹏 史玲 何锦风 王璋 |
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| 作者单位： | 1. 江南大学食品学院,江苏无锡,214122
2. 中国人民解放军总后勤部军需装备研究所军用汉麻材料研究中心,北京,100000 |
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| 摘 要： | 采用不同蛋白酶酶解汉麻籽蛋白,确定Alcalase 2.4L碱性蛋白酶是酶解汉麻籽蛋白制备抗氧化肽的优良酶源。通过单因素和响应面回归分析,得到Alcalase 2.4L碱性蛋白酶酶解汉麻籽蛋白的优化条件为:底物浓度50 mg/mL、水解时间2 h、温度50℃、加酶量2.2%、pH 9.4。优化酶解条件下,水解度约为20%,10 mg/ mL酶解产物的DPPH自由基清除率为82.65%,显示出较好的抗氧化活性。
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| 关 键 词： | 汉麻籽蛋白 酶法 抗氧化肽 响应面分析法 DPPH自由基 |
| 修稿时间： | 2007-10-09 |
Preparation of Antioxidant Peptides from Hempseed Protein by Enzymatic Hydrolysis |
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| Zhou Xuhui,Qian Ping,Lu Rongrong,Chen Tianpeng,Shi Ling,He Jinfeng,Wang Zhang. Preparation of Antioxidant Peptides from Hempseed Protein by Enzymatic Hydrolysis[J]. Food and Fermentation Industries, 2008, 34(5): 76-81 |
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| Authors: | Zhou Xuhui Qian Ping Lu Rongrong Chen Tianpeng Shi Ling He Jinfeng Wang Zhang |
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| Abstract: | Hempseed protein was hydrolyzed with four different enzymes.It was found that Alcalase2.4L alkaline protease had the best scavenging effect to DPPH.Using response surface regression analysis,we achieved the optimum enzyme hydrolysis conditions of Alcalase 2.4L alkaline protease as:substrate([S])50 mg/mL,time2 h,temperature(T)50℃,enzyme:substrate ratio([E]/[S])2.2%,and pH 9.4.The ob- tained degree of hydrolylsis(DH)was about 20%,the antioxidant activity of 10 mg/mL hydrolysate was a- bout 82.65%,which showed high antioxidant activity. |
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| Keywords: | hempseed protein enzymatic hydrolysis antioxidant peptide response surface method DPPH |
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