采用高通量微生物法和HPLC法检测猪肉中四环素和磺胺类抗生素残留

建立了一种采用高通量微生物法进行筛检,再用反相高效液相色谱法同时测定猪肉中土霉素、四环素、金霉素、磺胺甲基嘧啶、磺胺二甲嘧啶和磺胺间甲氧嘧啶的分析方法。样品取汁在96孔微板上采用嗜热脂肪芽孢杆菌(Bacillus sterothermophilus)进行筛检,同时采用Mcllvaine-EDTA溶液提取,选择270 nm和360 nm为检测波长进行检测。结果表明:所建立的微生法的检出限为0.02～0.05μg/mL,HPLC法的检出限为0.03～0.06 mg/kg,添加回收率80.25%～118.60%。

Analysis of Tetracycline and Sulfonamide Antibiotic Residues in Pig Muscle Using Throughout Microbial Assay and HPLC

A new protocol to analyze tetracyclines and sulfonamides from pig muscle was developed.Six antibiotics,oxytetracycline,tetracycline,chlortetracycline,slfamonomethoxine,sulfamethazine,and sulfamerazine,were analyzed by microbial assays and HPLC.Muscle juice samples were screened with Bacillus sterothermophilus in 96-well microplate.At the same time,the muscles samples were extracted with Mcllvaine-EDTA buffer solutions and then were detected at 270nm and 360nm.The detection limits of microbial assay were 0.02~0.05μg/mL.For HPLC method,the limits of detection were 0.03~0.06 mg/kg,and the average recoveries were 80.25%~118.60%.