Biological Control of Rambutan Post-Harvest Anthracnose (Colleotrichum gloeosporioides) by Combined Treatment of Trichoderma harzianum-TrH40 Culture Filtrates and Calcium Salts |
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Authors: | FMMT Marikar D Sivakumar RS Wilson Wijerathnam |
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Affiliation: | 1. Industrial Technology Institute , Sri Lanka;2. Sri Lankan Institute of Information Technology , Malabe, Sri Lanka faiz.m@sliit.lk;4. Industrial Technology Institute , Sri Lanka |
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Abstract: | Trichoderma harzianum-TrH40 isolated from soil samples from rambutan (Nephelium lappaceum) orchards had antagonistic effects with combination of CaCl2 salts on growth, activity, and infection of rambutan fruits by anthracnose (Colleotrichum gloeosporioides). Lower growth rate of spore germination, radial mycelial growth, and infectivity of anthracnose pathogen was observed at higher molar concentration of CaCl2 by in vitro studies. Germination rate was 0.5%?when 1M CaCl2 was combined with T. harzianum-TrH40 spore suspension. The disease incidence and severity of anthracnose were least on wounded fruits that had been dipped in a solution containing of 1,100 mg of CaCl2 per liter and T. harzianum-TrH40, which contained 106 spores/ml. Furthermore, disease incidence and severity with combined treatments were correlated significantly and effectively reduced infection in vivo in rambutan fruits. |
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Keywords: | Trichoderma harzianum anthracnose Colleotrichum gloeosporioides CaCl2 rambutan Nephelium lappaceum post harvest |
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