Detection of Cronobacter species in powdered infant formula by probe-magnetic separation PCR |
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| Authors: | Feng Xu Peng Li Xing Ming Dong Yang Hengyi Xu Xiaoli Wu Nagendra P Shah Hua Wei |
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| Affiliation: | * State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China;† Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China;‡ Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong |
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| Abstract: | Cronobacter species are opportunistic foodborne pathogens associated with serious infections in preterm neonates and infants. Based on the epidemiological research, infant formula products are considered to be the main source of infections from this organism. Therefore, accurate methods are required for detection of Cronobacter species. In this study, the specific probe and primers for detection of this organism were designed and verified. The probe-magnetic beads were prepared for sequence capture, followed by PCR assay to detect the target gene. This probe-magnetic separation PCR assay could detect as few as 103 cfu/mL of Cronobacter in artificially contaminated infant formulas in less than 4 h. The combination of magnetic beads and PCR showed the potential for the detection of Cronobacter in infant formulas and may have applications in the dairy industry. |
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| Keywords: | Cronobacter species probe-magnetic separation PCR powdered infant formula |
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