响应面法优化玛咖酰胺提取工艺的研究

以玛咖粉为原料,经酶预处理后,优化玛咖酰胺的提取工艺。通过单因素试验选取提取温度、提取时间、乙醇浓度3个因素,采用Box-Behnken响应面优化玛咖酰胺的提取工艺,建立二次多项式回归模型。结果表明,3个因素对总玛咖酰胺提取率的影响大小次序为:提取温度提取时间乙醇浓度;总玛咖酰胺的最佳提取条件为:提取温度71℃、提取时间2.7h、乙醇浓度95%。在此最佳工艺条件下,玛咖酰胺的提取率为80.87%,回归模型的预测值和实测值相对误差为0.11%。

Optimized Extraction of Macamides from Maca by Response Surface Methodology ZHENG Yue1,LU Yi1,HUI Ai-ling1,WU Ze-yu1,DU Biao2,ZHANG Hua-wei2,ZHANG Wen-cheng1

The optimum extraction condition of total macamides from maca powder by enzymatic pre-treatment. Three factors such as extraction temperature,extraction time,ethanol concentration were selected based on single factor experiment. The extraction process of alkaloids was optimized by Box-Behnken response surface methodology and developed a quadratic polynomial regression model to optimize the extraction conditions. The order of influence of the factors was:extraction temperature > extraction time >ethanol concentration. The optimum extraction parameters ware selected as follows:extraction temperature was 71℃,extraction time was 2.7h,ethanol concentration was 95%. It was validated that the extraction rate of total macamides was 80.87% in this condition,and the relative error between the predicted value and the measured value of the regression model was 0.11%.