| HPLC 法测定葛根超细粉中葛根素含量 |
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| 引用本文： | 任少伟,张文成,李兵,董秀丽,李如福,李学彬,李广龙.HPLC 法测定葛根超细粉中葛根素含量[J].包装与食品机械,2014(3):67-69. |
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| 作者姓名： | 任少伟 张文成 李兵 董秀丽 李如福 李学彬 李广龙 |
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| 作者单位： | 合肥工业大学农产品生物化工教育部工程研究中心;安徽信屹康中药工业有限公司; |
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| 摘 要： | 建立一种测定葛根超微粉中葛根素含量的方法。采用RP-HPLC法测定葛根素的含量。色谱柱为Agilent SGE protecol C18（5μm 4．6×250mm），流动相为甲醇-水（25∶75），流速为1．0mL/min，柱温为35℃，检测波长为250nm。葛根素在47～470μg/mL的线性关系良好，R＝0．9999。该方法操作简便、稳定可靠，可用于葛根超微粉的葛根素含量测定。
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| 关 键 词： | 葛根素 超微粉 含量测定 |
Studies on the Content Determination of Puerarin in Roots of Puerarin Lobata Superfine Powder by HPLC |
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| REN Shao-wei,ZHANG Wen-cheng,LI Bing,DONG Xiu-li,LI Ru-fu,LI Xue-bin,LI Guang-long.Studies on the Content Determination of Puerarin in Roots of Puerarin Lobata Superfine Powder by HPLC[J].Packaging and Food Machinery,2014(3):67-69. |
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| Authors: | REN Shao-wei ZHANG Wen-cheng LI Bing DONG Xiu-li LI Ru-fu LI Xue-bin LI Guang-long |
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| Affiliation: | 1. Engineering Research Center of Bio-process from Ministry of Education, Hefei University of Technology, Hefei 230009, China ;2. Anhui Xinyikang Traditional Chinese Medicine Industry Co. Ltd. , Hefei 230001, China) |
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| Abstract: | This study has been to establish a method of determination of puerarin in roots of Puerarin loba -ta superfine powder.SGE protecol C18 （5μm,4.6 ×250mm） chromatographic column was used ,with mix-tures of methanol and water as mobile phase （25∶75,v/v）,the flow rate was 1.0mL/min,column temperature was 35℃,the wavelength was 250nm,the injection volume was 20 μL.The linear correlation of puerarin is good in range of 47~470μg/ml,the correlation coefficient r2 is 0.9998.The presented method is simple and reliable for determining of roots of Puerarin lobata superfine powder . |
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| Keywords: | puerarin superfine powder content determination |
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