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羊肚菌液体深层发酵工艺优化研究
引用本文:汪梦筱,王伟,汪维云.羊肚菌液体深层发酵工艺优化研究[J].包装与食品机械,2014(1):15-18.
作者姓名:汪梦筱  王伟  汪维云
作者单位:安徽农业大学生命科学学院;
基金项目:安徽农业大学大学生科技创新基金项目(项目号:2012266)
摘    要:为了探讨羊肚菌的液体发酵工艺,优化液体深层发酵培养基的配方。在单因素试验的基础,进行了多因素正交试验。结果表明,羊肚菌的液体深层发酵培养基最佳配方为:葡萄糖1.5%、玉米粉1.5%、蛋白胨0.3%、黄豆粉0.3%、KH2 PO40.3%、MgSO40.2%、CaCO30.1%、VB10.001%,菌丝生物量达3.10 g·100mL-1;液体培养条件为pH值6.5左右、温度25℃。

关 键 词:羊肚菌  深层培养  工艺  菌丝生物量

Studies on Optimization Technology of Submerged Fermentation for Morehella esculenta
WANG Meng-xiao,WANG Wei,WANG Wei-yun.Studies on Optimization Technology of Submerged Fermentation for Morehella esculenta[J].Packaging and Food Machinery,2014(1):15-18.
Authors:WANG Meng-xiao  WANG Wei  WANG Wei-yun
Affiliation:WANG Meng-xiao, WANG Wei, WANG Wei-yun
Abstract:In order to discuss technology of submerged fermentation of Morchella,the submerged culture medium formula was optimized.Based on single factor experiment,the orthogonal test was made.It is shown that the best formula were glucose 1 .5%,corn powder 1 .5%,peptone 0.3%,soybean powder 0.3%,KH2 PO4 0.3%,MgSO4 0.2%,CaCO3 0.1%,VB1 0.001%,the mycelium biomass could reach 3.1 0 g·1 00mL-1 .The submerged culture conditions were temperature 25℃ and pH 6.5 for Morehella esculenta.
Keywords:Morehella esculenta  submerged culture  technology  mycelium biomass
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