固相萃取-高效液相色谱-串联质谱法测定番茄酱中棒曲霉素

采用固相萃取-高效液相色谱-串联质谱(SPE-HPLC-MS/MS)建立了番茄酱中的棒曲霉素的定性定量分析方法。样品经Strata-X固相萃取净化处理,使用Agilent XDB-C18色谱柱(50mm×2.1mm,1.8μm)、以乙腈-水为流动相梯度洗脱,在流速为0.12mL/min条件下分离,采用电喷雾离子化源(ESI)多反应监测(MRM)模式下检测。在最佳检测条件下,棒曲霉素在0.05～0.5mg/L检测范围内线性关系良好,相关系数大于0.9995;检出限为0.03mg/L、定量限为0.1mg/L,该方法的精密度小于1.5%,回收率在90.2%～95.6%之间。说明该法准确可靠,可应用于番茄酱中棒曲霉素含量的测定。

Determination of Patulin by Solid-phase Extraction-high Performance Liquid Chromatography-tandem Mass Spectrometry in Tomato Sauce

A method was established for the determination of Patulin by solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry(SPE-HPLC-MS/MS) in tomato sauce.The sample clean-up with Strata-X solid phase extraction,and separations were performed on an Agilent SB-C18 column(50 mm×2.1 mm,1.8 μm) with a mobile phase of acetonitrile-water at a flow rate of 0.12 mL/min and detected by electrospray ionization MS/MS under the under multiple reaction monitoring(MRM) mode.The results showed it were obtained that the good linear relationships and the correlation coefficients greater than 0.9995.The limits of detection(LOD) was 0.03 mg/L,the limits of quantification(LOQ) was 0.1 mg/L.The precision was less than 1.5%,the recovery rate was between the 90.2%~95.6% of the method.Indicating that the method is accurate and can be used for the determination of patulin in tomato sauce.