QuEChERS萃取-UPLC-MS/MS测定花生酱中黄曲霉毒素B1方法的研究

建立了QuEChERS萃取-UPLC-MS/MS测定花生酱中黄曲霉毒素B1的快速检测方法。样品首先经过1%甲酸-乙腈溶液提取,提取液采用QuEChERS净化试剂(250mg MgSO4+100mg HC-C18+50mg PSA)净化后上机,UPLC-MS/MS正离子源多反应模式检测,外标法定量。黄曲霉毒素B1在1.0~5.0ng/mL范围内呈良好线性关系,相关系数(R^2)>0.999,4个水平的添加目标分析物黄曲霉毒素B1的回收率在81.7%~93.5%范围内,相对标准偏差(RSD)为2.4%~5.6%,检出限为0.03μg/kg;该试验方法具有简单、高效、经济、准确、回收率高、精密度好的优点,适用于花生酱样品中黄曲霉毒素B1的快速检测。

Study on Determination of Aflatoxin B1 in Peanut Butter by QuEChERS Extraction and UPLC-MS/MS

A rapid detection method of aflatoxin B1 in peanut butter by QuEChERS extraction-UPL-CMS/MS is established.The sample is extracted by 1% formic acid-acetonitrile solution.The extract is purified by QuEChERS purification reagent(250 mg MgSO4+100 mg HC-C18+50 mg PSA).The UPLC-MS/MS positive ion source is detected by multi-reaction mode and quantified by external standard method.Aflatoxin B1 shows a good linear relationship in the range of 1.0~5.0 ng/mL,the correlation coefficient(R^2)is>0.999.The recovery rate of aflatoxin B1 in target analyte at four levels ranges from 81.7%to 93.5%,the relative standard deviation(RSD)is 2.4%~5.6%,and the detection limit is 0.03μg/kg.This method has the advantages of simplicity,rapidity,economy,accuracy,high recovery rate and good precision,and it is suitable for the rapid detection of aflatoxin B1 in peanut butter samples.