Evaluation of Ammonia Pretreatment for Enzymatic Hydrolysis of Sugarcane Bagasse to Recover Xylooligosaccharides |
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| Authors: | Sosyu Tsutsui Kiyoshi Sakuragi Kiyohiko Igarashi Masahiro Samejima Satoshi Kaneko |
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| Affiliation: | 1. Department of Subtropical Biochemistry and Biotechnology, Faculty of Agriculture, University of the Ryukyus ; 2. Energy Engineering Research Laboratory, Central Research Institute of Electric Power Industry ; 3. Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo ; 4. Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University |
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| Abstract: | Sugarcane bagasse is a useful biomass resource. In the present study, we examined the efficacy of ammonia pretreatment for selective release of hemicellulose from bagasse. Pretreatment of bagasse with aqueous ammonia resulted in significant loss of xylan. In contrast, pretreatment of bagasse with anhydrous ammonia resulted in almost no xylan loss. Aqueous ammonia or anhydrous ammonia-pretreated bagasse was then subjected to enzymatic digestion with a xylanase from the glycoside hydrolase (GH) family 10 or a xylanase from the GH family 11. The hydrolysis rate of xylan in bagasse pretreated with aqueous ammonia was approximately 50 %. In contrast, in the anhydrous ammonia-treated bagasse, xylan hydrolysis was > 80 %. These results suggested that anhydrous ammonia pretreatment would be an effective method for preparation of sugarcane bagasse for enzymatic hydrolysis to recover xylooligosaccharides. |
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| Keywords: | Ammonia pretreatment, sugarcane bagasse, hemicellulose, xylan, β -xylanase |
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