Cell cycle analysis of Chinese hamster ovary cells stimulated by phosphatidic acid in serum-free culture |
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| Authors: | Yamaji Hideki Sakai Kentaro Joho Tsukasa Izumoto Eiji Fukuda Hideki |
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| Affiliation: | Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. yamaji@kobe-u.ac.jp |
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| Abstract: | When recombinant Chinese hamster ovary cells were treated with pertussis toxin or genistein, not only lysophosphatidic acid (LPA) but also phosphatidic acid (PA) failed to stimulate progression through the cell cycle in serum-free culture, suggesting that PA and LPA induce cell growth through the same signal transduction pathway. Cell cycle analysis also indicates that cell growth promoted by PA results in enhanced protein production. |
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| Keywords: | phosphatidic acid Chinese hamster ovary (CHO) cells serum-free culture cell cycle analysis flow cytometry |
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