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Antibiotic resistance,biochemical typing,and PFGE typing of <Emphasis Type="Italic">Bifidobacterium</Emphasis> strains commonly used in probiotic health foods
Authors:Feili Xu  Junping Wang  Yunchang Guo  Ping Fu  Huawei Zeng  Zhigang Li  Xiaoyan Pei  Xiumei Liu  Shuo Wang
Affiliation:1.Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology,Ministry of Education,Tianjin,China;2.National Food Safety Risk Assessment Center,Beijing,China;3.Department of Bioengineering, College of Life Sciences,Huaibei Normal University,Huaibei,China
Abstract:This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemical-type strain also had similar antibiotic resistance patterns.
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