Optimum conditions for the enzymatic hydrolysis of citron waste juice using response surface methodology (RSM) |
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Authors: | Ji Young Lim Hyang-Sik Yoon Kwang-Yup Kim Ki-Sik Kim Jae Goan Noh In Gyu Song |
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Affiliation: | (1) School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-cho, Nobeoka 882-8508, Japan;(2) Department of Pharmacy, Miyazaki Medical College Hospital, 5200 Kihara, Kiyotake-cho, Miyazaki-gun, Miyazaki 889-1692, Japan;(3) Department of Nutrition Science, Faculty of Health and Nutrition Sciences, Minami Kyushu University, 5-1-2 Kirishima, Miyazaki 880-0032, Japan |
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Abstract: | This study investigated the optimum conditions, including pH, temperature, incubation time, and enzyme concentration, for
the enzymatic manufacture of citron waste juice by pectinase and cellulase using response surface methodology (RSM). A central
composite design was used as an experimental design for the allocation of treatment combination. The highest yield was 71.1%
at pH 5.00, 51.15°C, 53.74 min, and 0.44%, whereas the lowest turbidity was 0.20 at pH 4.87, 50.21°C, 49.99 min, and 0.44%.
The highest hunter’s L (lightness), a (redness), and b- (yellowness) values were 47.39 (pH 4.86, 50.72°C, 51.60 min, and 0.44%),
−1.34 (pH 5.48, 49.36°C, 46.29 min, and 0.21%), and 13.18 (pH 5.49, 50.05°C, 43.31 min, and 0.23%), respectively. The highest
electron donating activity was 68.36% at pH 4.51, 50.62°C, 47.00 min, and 0.23%. The highest limonin and nomilin contents
were 3.49 mg/100 g (pH 4.51, 50.30°C, 48.34 min, and 0.21%) and 1.56 mg/100 g (pH 4.59, 50.08°C, 66.07 min, and 0.30%). |
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