Cytoprotective activity of extract of roasted coffee residue on mouse embryonic fibroblasts cells against apoptosis induced by oxidative stress |
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Authors: | Jung-Suk Sung Eun-Bi Go Han-Seung Shin |
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Affiliation: | 2. Department of Life Science, Dongguk University-Seoul, Seoul, 100-715, Korea 1. Department of Food Science and Biotechnology and Institute of Lotus Functional Food Ingredients, Dongguk University-Seoul, Seoul, 100-715, Korea
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Abstract: | This study was conducted to evaluate the cytoprotective activity of roasted coffee residues (RCRs) extract on mouse embryonic
fibroblast (MEF) cells. RCRs originated from Colombia and Honduras are relatively nontoxic to cell growth and even stimulate
cell proliferation. Colombian RCRs showed most efficient protective effects on MEF cells against oxidative damage induced
by H2O2 compared among the extracts prepared under the same roasting time. The most significant radical scavenging activity was measured
in RCR with roasting time of 8.5 min. Phenolic and nonphenolic compounds in RCRs were chlorogenic acid, caffeine, caffeic
acid, nicotinic acid, trigonelline, and 5-(hydroxymethyl)furfuralolehyde. Effect of Colombian RCRs on apoptosis occurred by
oxidative damage was evaluated by morphological and flow cytometric analysis. Apoptotic cell accumulation was decreased by
cotreatment of MEF cells with Colombian RCRs. These results suggested that antioxidant potency of RCRs suppresses the cytotoxicity
which is induced by H2O2 and has a protective effect on MEF cell against oxidative stress. |
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