卟啉接枝细菌纤维素的制备及其光敏抗菌性能

针对医院获得性感染细菌耐药性的问题，在细菌纤维素（BC）表面接枝卟啉制备了一种光敏抗菌材料。首先将二醛细菌纤维素浸渍在高碘酸钠（NaIO4 ）中制备得到二醛细菌纤维素（BC-CHO），然后利用还原胺化反应在BC-CHO表面接枝乙二胺（EDA），最后共价接枝活化的原卟啉（PpIX）合成得到乙二胺化细菌纤维素原卟啉纤维膜。借助傅里叶变换红外光谱仪、热重分析仪、X 射线衍射仪、扫描电子显微镜对纤维膜的性能进行分析。结果表明：二醛细菌纤维素表面成功地接枝了氨基间隔臂乙二胺和PpIX；随着NaIO4 浓度的提高，BC-CHO 醛基含量逐渐提高，热分解温度逐渐降低，晶体结构逐渐发生转变；纤维膜对金黄色葡萄球菌的初次抗菌率可达到98%，经过5次酒精洗涤后仍然具有良好的抑菌性能。

Preparationof porphyrin grafted bacterial cellulose andphotodynamic antimicrobial property thereof

In view of the drug-resistant characteristic of nosocomial bacterial infection, a novelphotosensitive antimicrobial material pripared bygrafting porphyrin were synthesized.Oxidized bacterial cellulose (BC-CHO) was prepared by immersing in sodium periodate (NaIO4) firstly, then ethylenediamine (EDA) was grafted onto the bacterial cellulose by reductive amination reaction, and finally protoporphyrin IX (PpIX) grafted ethylenediamined bacterial cellulose fibrous membrane was obtained by covalenting activated PpIX onto as-obtained ethylenediamined bacteria cellulose. Fourier transform infrared spectrometer, thermogravimetric analyzer,X-ray diffraction and scanning electron microscopy were utilized to analyze the functional groups, thermal properties, crystal structure of as-grafted fibrous membranes. At last, antibacterial behavior of BC-EDA-PpIX fibrous membrane and its reusability were discussed. The results show that amino spacer arm as well as the the a PpIX is successfullygrafted onto the dialdehyde bacterial cellulose. Furthermore, the aldehyde content of BC-CHO gradually increased with the increase of NaIO4 concentration. Meanwhile, the thermal decomposition temperature decreased gradually, and the crystallinity slightly decreases. The first-timed antibacterial effect of the as-synthesized BC-EDA-PpIX fibrous membrane againse Staphylococcus aureus reached 98%, and such material still shows good antibacterial activity even after 5 times of ethanol washing.