酪氨酸酶酶促乳铁蛋白在丝素表面的接枝

利用酪氨酸酶对酪氨酸残基具有催化氧化的特性,进行酶促乳铁蛋白在丝素表面接枝效果研究。借助酪氨酸含量分析、丝素蛋白溶液黏度测定等,评价了酪氨酸酶的催化接枝效果。结果表明,经酪氨酸酶处理后,丝纤维中酪氨酸含量下降;与仅经乳铁蛋白处理相比,酪氨酸酶/乳铁蛋白组合后丝织物的强力、丝素蛋白溶液黏度增加,验证了酶促乳铁蛋白在丝素蛋白表面的接枝反应。此外,乳铁蛋白也会在丝纤维表面吸附,表现为酪氨酸酶/乳铁蛋白处理样,与仅乳铁蛋白处理样染色性能较相近。

Tyrosinase-catalyzed modification of silk fibroin by grafting of lactoferrin

Tyrosinases have the potential abilities of catalytic oxidation of tyrosine residues. In this paper, the efficacy of the enzymatic grafting of lactoferrin onto silk fibroin with tyrosinase was investigated. The changes of tyrosine content in silk fibroin and the relative viscosity of silk fibroin solution were also evaluated. The results indicated that the content of tyrosine in silk fibroin decreased after incubating with tyrosinase. For the sample combinedly treated with tyrosinase and lactoferrin, the tensile strength of the silk fabric and its relative viscosity of fibroin solution seemed high as compared to that of the sample treated with loctoferrin alone, which verified the occurrence of the enzymatic grafting reaction on silk surface. Further, the loctoferrin might simultaneously adsorb onto fibroin during the mentioned enzymatic grafting, the color depth of the silk fabric treated with tyrosinases/loctoferrin was similar to that treated with loctoferrin alone.