黄麻纤维中黄酮类化合物提取工艺优化及表征

为充分开发黄麻纤维的应用价值,探究其中是否含有黄酮类化合物,采用水醇提取法制备了黄麻纤维提取液,利用紫外分光光度法测试提取液中黄酮含量,按照二次通用旋转组合设计方法对提取工艺进行优化,研究了乙醇体积分数、浴比、提取时间3个工艺因子与黄酮类化合物提取率之间的关系。对经过旋转蒸发和真空冷冻干燥后黄麻纤维提取物的化学结构、热稳定性和表面形貌进行测试与分析。结果表明:黄麻纤维提取物与芦丁标准品化学结构相似,说明其中含有黄酮类化合物,其最优提取工艺是乙醇体积分数75%,浴比1∶50,提取时间150 min, 该条件下黄酮的提取率为0.037%。

Optimization and characterization of flavonoids extracted from jute fiber

In order to confirm the existence of flavonoids in jute fiber and to develop the application value, the jute fiber extracts were obtained using alcohol-water method. The content of flavonoids in the extraction solution was tested using ultraviolet spectrophotometer. The extraction process was optimized based on the use of quadratic general rotary design, and the relationships between the three factors(alcohol volume fraction, bath ratio and extracting time) and the extraction rate of flavonoids were studied. The chemical structure, thermal stability and morphology of jute fiber extracts after rotary evaporation and vacuum freeze drying were tested and analyzed. The results show that the chemical structure of jute fiber extracts is similar to the structure of rutin standard, which indicates that it contains flavonoids. The optimal extraction conditions were found to be ethanol volume fraction 75%, bath ratio 1∶50, and extracting time 150 min, at which the extraction rate of flavonoids was 0.037%.