超临界CO2介质的苎麻酶法脱胶研究初探

 在35~55℃、7~15 MPa条件下,利用果胶粗酶液和木聚糖酶液,对苎麻韧皮进行超临界CO2处理及其脱胶试验,考察了超临界CO2处理苎麻韧皮前后酶液中的活菌数、酶活和脱胶效果。实验结果表明,经超临界CO2处理1 h左右,苎麻脱胶菌的致死率在99%以上;不同类型非纤维素降解酶,在超临界条件下的稳定性不同,木聚糖酶比较稳定,酶活仅降低1.6%,而果胶粗酶液的稳定性较差,酶活降低23.79%;超临界CO2介质有利于加速苎麻酶的脱胶催化反应进程,使苎麻脱胶效果提高60%~100%。

Preliminary study on ramie retting in supercritical-CO2 with enzymes

This paper describes an experiment of ramie bast retting treatment with pectinase and xylanase preparations under the conditions of supercritical CO_2 at 35～(55 ℃),7～(15 MPa,) and investigates the survival number of organisms,variations of enzyme activity and the retted effect before and after the treatment.The results demonstrate that the inactivity rate of retting organisms is over 99% under the condition of supercritical CO_2 after one hour.Under supercritical conditions,the stability of non-cellulase varies with its species,xylanase is comparatively stable and its activity decreases by only 1.6%,and pectinase stability is,however,not so good and its activity decreases by 23.79%.The enzymatic retting of ramie in the medium of supercritical CO_2 is beneficial to accelerate the catalytic process and the retting efficiency is increased by 60%～100%.