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骆驼乳对慢性酒精性肝损伤小鼠肠道菌群的影响
引用本文:白路平,乔向宇,海勒,其布勒,吉日木图,明亮. 骆驼乳对慢性酒精性肝损伤小鼠肠道菌群的影响[J]. 中国食品学报, 2022, 22(1): 78-87
作者姓名:白路平  乔向宇  海勒  其布勒  吉日木图  明亮
作者单位:内蒙古农业大学 乳品生物技术与工程教育部重点实验室 呼和浩特 010018;内蒙古农业大学 乳品生物技术与工程教育部重点实验室 呼和浩特 010018;内蒙古骆驼研究院 内蒙古阿拉善 750306
基金项目:国家重点研发计划项目(2020YFE0203300);内蒙古自治区科技成果转化专项(AMCG201911);高层次人才引进科研启动项目(NDYB2017-28);双一流学科创新团队建设项目(NDSC2018-14);内蒙古自然科学基金项目(2018BS03017) 作者简介:白路平(1994—),女,硕士生
摘    要:目的:探究骆驼乳对慢性酒精性肝损伤小鼠肠道菌群多样性及结构的影响。将雄性C57BL/6NCr小鼠随机分为4组:对照组(Con,n=6)、模型组(Et,n=6)、骆驼乳剂量组(EtCM,n=6)和牛乳剂量组(EtNM,n=6);实验期为8周,前4周饲喂Lieber-DeCarli液体饲料(含对照),后4周在饲喂Lieber-DeCarli液体饲料的基础上,灌胃相应的乳或生理盐水。灌胃结束后,按照5 g/kg剂量一次性灌胃31.5%酒精溶液,建立NIAAA模型。检测血清LPS含量,并在无菌条件下取小鼠结肠粪便,进行16S rRNA测序,分析肠道菌群α多样性、β多样性及基于门、属水平的物种结构。血清指标结果显示,EtCM组和EtNM组小鼠血清LPS显著降低(P<0.01)。16S r RNA测序结果表明,骆驼乳和牛乳能显著提高ALD小鼠结肠肠道菌群的丰度和均匀度,更好地调整肠道菌群结构,其中骆驼乳较牛乳显示出更好的α多样性。在门水平上,骆驼乳和牛乳显著提高拟杆菌门的丰度,降低厚壁菌门的丰度。在属水平上,骆驼乳和牛乳显著提高副拟杆菌属、拟杆菌属、阿克曼菌属的丰度,降低瘤胃菌科下的未知属RuminococcaceaeUCG-013丰度。其中,骆驼乳的有益菌丰度较牛乳高出9%。结论:骆驼乳通过改变ALD小鼠肠道菌群环境,来调节肠道菌群结构,可作为调节肠道菌群的功能性乳制品,可预防慢性ALD引起的肠道屏障功能障碍。

关 键 词:骆驼乳  16S  rRNA  慢性酒精性肝损伤  肠道菌群
收稿时间:2020-12-30

Effect of Camel Milk on Intestinal Flora of Mice with Chronic Alcoholic Liver Injury
Bai Luping,Qiao Xiangyu,Hai Le,Qi Bule,Jirimutu,Ming Liang. Effect of Camel Milk on Intestinal Flora of Mice with Chronic Alcoholic Liver Injury[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22(1): 78-87
Authors:Bai Luping  Qiao Xiangyu  Hai Le  Qi Bule  Jirimutu  Ming Liang
Affiliation:Key Laboratory of Dairy Biotechnology and Bioengineering,Ministry of Education,Inner Mongolia Agriculture University,Hohhot 010018;Key Laboratory of Dairy Biotechnology and Bioengineering,Ministry of Education,Inner Mongolia Agriculture University,Hohhot 010018;Inner Mongolia Institute of Camel Research,Alxa 750306,Inner Mongolia
Abstract:Purpose:To explore the effect of camel milk on the diversity and structure of intestinal microflora in mice with chronic alcoholic liver injury.In this experiment,male C57BL/6NCr mice were randomly divided into 4 groups:control group (Con,n=6),model group (Et,n=6),camel milk dose group (EtCM,n=6) and cow milk dose group (EtNM,n=6);the experiment was conducted for 8 weeks,the first 4 weeks were fed with Lieber-DeCarli liquid feed (including control),and the following 4 weeks were fed with corresponding milk or saline on the basis of feeding Lieber-DeCarli liquid feed.After the gavage,the NIAAA model was established by gavage 31.5% alcohol solution at a dose of 5 g/kg.Serum LPS content was detected,and colonic feces of mice were collected under sterile conditions for 16S rRNA sequencing,and intestinal flora alpha diversity,beta diversity,and species structure based on genus and genus levels were analyzed.Serum index results showed that serum LPS of mice in EtCM group and EtNM group was significantly reduced (P < 0.01).16S rRNA sequencing results showed that camel milk and cow milk significantly increased the abundance and uniformity of the colonic intestinal flora of ALD mice,and better adjusted the structure of intestinal flora;among them,camel milk showed better alpha diversity than cow milk.At the phylum level,camel milk and cow milk significantly increased the abundance of Bacteroides phylum and decreased the abundance of thick-walled phylum.At the genus level,camel milk and cow milk significantly increased the abundance of Bacteroides,Bacteroides,and Ackermania,and reduced the abundance of the unknown genus Ruminococcaceae_UCG-013 under the Rumenaceae family.Among them,the beneficial bacteria abundance of camel milk is 9% higher than that of cow milk.Conclusion:Camel milk can adjust the intestinal flora structure by changing the intestinal flora environment of ALD mice.It shows that camel milk can be used as a functional dairy product to regulate intestinal flora,and can prevent intestinal barrier dysfunction caused by chronic ALD.
Keywords:camel milk  16S rRNA  chronic alcoholic liver injury  intestinal flora
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