HPLC测定乳制品中的甲醛含量

针对奶糖及奶制品的甲醛残留问题,建立了一种灵敏、快速和有效的HPLC-UV检测乳制品中甲醛的方法。牛奶和奶粉样品中甲醛经三氯乙酸(TCA)提取,奶糖另添加乙酸锌和亚铁氰化钾进行辅助沉淀,用1mg/mL2,4-二硝基苯肼(DNPH),在60℃条件下衍生30min后,用HPLC-UV分离测定,外标法定量。该方法对3种奶制品平均加标回收率在95%以上,日内精密度和日间精密度在5%以下,甲醛含量在0.03～4.00μg/mL范围内呈良好的线性关系,检出限和定量限分别为0.03μg/mL和0.10μg/mL,对常见醛类和醇类有较好的抗干扰性。该方法具有操作简单,准确性高,稳定性好,抗干扰性强的特点,是一种测定乳制品中甲醛的有效分析技术。

Determination of Formaldehyde in Milk Product by HPLC

A high-performance liquid chromatography(HPLC)-ultraviolet(UV) method was developed for determination of formaldehyde in different milk products,in order to investigate the residues of formaldehyde in various sugar and milk products.Samples were based on trichloroacetic acid(TCA),Zn(AC)2 and K4Fe(CN)6 pertreatment,formaldehyde was derivated with 1 mg/mL 2,4-dinitrophenylhydrazine(DNPH) at 60 ℃ for 30 min,then analysis by HPLC at UV-detector.The average recovery for the three kinds of samples were above 95%.The both of with-day and betweenday precision were lower than 5%.A goodlinear relation was observed between 0.03～4.00 μg/mL of amout of formaldehyde.The LOD and LOQ were 0.03 and 0.10 μg/mL,respectively.The method had good anti-interference to similar chemicals of aldehyde and alcohol.Thus,the HPLC method,with a simpler operation,and a better accuracy and stability,was available to determine the formaldehyde in various milk products with satisfactory results.