首页 | 本学科首页   官方微博 | 高级检索  
     

河豚毒素DNA适配子的筛选与结构分析
引用本文:邵碧英,高兴,杨方,陈文炳,缪婷玉,彭娟.河豚毒素DNA适配子的筛选与结构分析[J].中国食品学报,2012,12(2):137-143.
作者姓名:邵碧英  高兴  杨方  陈文炳  缪婷玉  彭娟
作者单位:1. 福建出入境检验检疫局 福州350001
2. 福建出入境检验检疫局 福州350001;福州大学化学化工学院 福州350002
摘    要:人工合成113个碱基的随机ssDNA文库,以河豚毒素为靶物质,采用SELEX技术进行12轮筛选,测定各轮富集文库与河豚毒素的亲和力,其中第10轮富集文库与河豚毒素的亲和力最高。将第10轮富集文库进行克隆、测序,用DNAMAN软件分析克隆子的一级和二级结构,并测定克隆子与河豚毒素的亲和力。结果表明:与河豚毒素结合力高的DNA适配子经过10轮筛选后得到富集,成功筛选到最高亲和力的DNA适配子G10。

关 键 词:河豚毒素  适配子  SELEX  诱变PCR

Screening and Structure Analysis of the Aptamer Against Tetrodotoxin
Shao Biying , Gao Xing , Yang Fang , Chen Wenbing , Miao Tingyu , Peng Juan.Screening and Structure Analysis of the Aptamer Against Tetrodotoxin[J].Journal of Chinese Institute of Food Science and Technology,2012,12(2):137-143.
Authors:Shao Biying  Gao Xing  Yang Fang  Chen Wenbing  Miao Tingyu  Peng Juan
Affiliation:1 (1Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001 2College of Chemistry and Chemical Engineering of Fuzhou University,Fuzhou 350002)
Abstract:An in vitro synthesized 113 mer random ssDNA library was subjected to 12 rounds of selection against tetrodotoxin using SELEX method.The affinities between the various round enrichment library and tetrodotoxin were detected.The affinity to tetrodotoxin of the tenth round enrichment library was highest,then the enrichment library was cloned and sequenced.The primary and secondary structures of the clones were analyzed by DNAMAN software,and the affinities between each clone and tetrodotoxin were detected.The results showed that the aptamers with high affinity to tetrodotoxin were enriched after ten round selecting and the aptamer G10 with the highest affinity was successfully selected from the initial random ssDNA pool.
Keywords:tetrodotoxin  aptamer  SELEX  mutagenic PCR
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号