| 酒精酵母生物合成1.6-二磷酸果糖的条件优化研究 |
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| 引用本文： | 董亚晨,冯宇,方若思,徐腾洋,刘晓杰,陈启和.酒精酵母生物合成1.6-二磷酸果糖的条件优化研究[J].中国食品学报,2011,11(8). |
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| 作者姓名： | 董亚晨 冯宇 方若思 徐腾洋 刘晓杰 陈启和 |
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| 作者单位： | 浙江大学食品科学与营养系 杭州310029 |
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| 摘 要： | 为优化酒精酵母F15菌株转化合成1,6-二磷酸果糖(FDP)的条件,在单因素试验基础上,采用Box-Benhnken设计响应面中心组合试验,建立FDP生物合成量随反应液初始pH值、葡萄糖浓度和磷酸二氢钠浓度变化的二次回归方程.结果表明:最适的转化条件为初始pH 8、葡萄糖11％、磷酸二氢钠7％,在此转化条件下,获得的最大FDP生物合成量为3.74 g/L.
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| 关 键 词： | 酒精酵母F15 1 6-二磷酸果糖 响应面法 优化 |
Optimization of the Biosynthetic Conditions on FDP Production by Yeast Strain F15 |
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| Dong Yachen,Feng Yu,Fang Ruosi,Xu Tengyang,Liu Xiaojie,Chen Qihe.Optimization of the Biosynthetic Conditions on FDP Production by Yeast Strain F15[J].Journal of Chinese Institute of Food Science and Technology,2011,11(8). |
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| Authors: | Dong Yachen Feng Yu Fang Ruosi Xu Tengyang Liu Xiaojie Chen Qihe |
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| Affiliation: | Dong Yachen Feng Yu Fang Ruosi Xu Tengyang Liu Xiaojie Chen Qihe* (Department of Food Science and Nutrition,Zhejiang University,Hangzhou 310029) |
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| Abstract: | The purpose of the research was to optimize the condition of biosynthesis of FDP by saccharomyces cerevisiae F15. The optimum pH, glucose and NaH2PO4 concentration was determined using single factor experiment. The condition was optimized by Box-Benhnken design, and a quadratic regression equation for the change of biological synthesis of FDP with pH, glucose and NaH2PO4 concentration was established. The result showed that the most reasonable transformation condition were pH 8, glucose 11%, NaH2PO4 7%. Und... |
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| Keywords: | Saccharomyces cerevisiae F15 FDP response surface methodology optimization |
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