| 油茶籽壳总黄酮的提取及抗氧化研究 |
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| 引用本文： | 姜天甲,应铁进,陈秋平,康海权,沈建福,.油茶籽壳总黄酮的提取及抗氧化研究[J].中国食品学报,2010,10(1):93-99. |
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| 作者姓名： | 姜天甲 应铁进 陈秋平 康海权 沈建福 |
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| 作者单位： | 浙江大学生物系统工程与食品科学学院,杭州,310029 |
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| 摘 要： | 研究油茶籽壳中总黄酮类物质的提取工艺,探讨影响提取率的主要因素,通过正交试验确定最佳提取工艺。通过DPPH法﹑FRAP法﹑.OH清除能力3个体系对不同溶剂提取物的体外抗氧化能力进行研究。试验结果表明:在30倍样重的60%乙醇中浸泡后,40℃下超声波辅助萃取45 min,油茶籽壳中的总黄酮提取率为1.71%。此外,不同溶剂提取物中黄酮提取率差异很大。在DPPH体系中,油茶籽壳60%乙醇提取物的IC50为781μg/mL,清除能力强于其它溶剂提取物与对照品竹叶提取物(IC50为1 025μg/mL,主要成分为竹叶黄酮)(P0.05);60%乙醇提取物的FRAP值为1.79 mmol/L,显示出与竹叶提取物相当的还原能力;在清除羟自由基(.OH)体系中,样品质量浓度为500μg/mL时,乙醇提取物的清除效果与竹叶提取物相当;当样品质量浓度为1 000μg/mL时,乙醇提取物的清除效果稍弱于竹叶提取物。
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| 关 键 词： | 油 茶籽壳 总黄酮 提取溶剂 抗氧化 |
Total Flavonoids and Antioxidant Activity of Different Solvent Extracts from Shells of Oiltea(Camellia oleifera) Seed |
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| Jiang Tianjia,Ying Tiejin,Chen Qiuping,Kang Haiquan,Shen Jianfu.Total Flavonoids and Antioxidant Activity of Different Solvent Extracts from Shells of Oiltea(Camellia oleifera) Seed[J].Journal of Chinese Institute of Food Science and Technology,2010,10(1):93-99. |
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| Authors: | Jiang Tianjia Ying Tiejin Chen Qiuping Kang Haiquan Shen Jianfu |
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| Affiliation: | Jiang Tianjia Ying Tiejin Chen Qiuping Kang Haiquan Shen Jianfu(College of Biosystem Engineering , Food Science,Zhejiang University,Hangzhou 310029) |
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| Abstract: | The extracting technology and the effect of main factors on the extracting rate of total flavones from the shell of oiltea(Camellia oleifera) seeds were studied,the optimum conditions were determined through orthographic experiments.The solvent extracts were screened for their potential antioxidant activities using tests such as 1,1-diphenyl-2-picrylhydrazyl(DPPH),OH,and FRAP in vitro model systems.The results showed that 30:1 of 60% ethanol with ul-trasonic treatment last for 45min could get flavones as hi... |
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| Keywords: | Oiltea(Camellia oleifera) shell total flavonoids extraction solvent antioxidant |
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