乳酸链球菌L16对感染金黄色葡萄球菌小鼠肠道菌群的调节作用

为研究乳酸链球菌L16对金黄色葡萄球菌感染小鼠肠道菌群结构的恢复情况,采用高通量测序技术,构建小鼠肠道微生物的16S rRNA基因测序文库,通过菌群结构图、等级丰度分布曲线、热图、稀释性曲线图、维恩图等,分析预防组、治疗组、正常组和模型组小鼠的肠道菌群组成和生物多样性。结果:模型组中脱铁杆菌门(12.67%)显著升高,菌群结构异常。预防组中厚壁菌门(49.03%)和拟杆菌门(43.15%)的相对丰度接近正常组(44.75%;42.08%)。结论:乳酸链球菌L16能预防金黄色葡萄球菌感染小鼠肠道,调节肠道菌群结构。

Regulatory Effects of Streptococcus lactis L16 on Intestinal Flora in Mice Infected by Staphylococcus aureus

This experiment studied the recovery of Streptococcus lactis L16 on mice intestinal flora of Staphylococcus aureus after infection.The 16S rRNA gene sequencing library of mouse intestinal microbiota was constructed by high throughput sequencing technique,the composition and biodiversity of the prevention group,the treatment group,the control group and the model group were analyzed,by the bacterial community structures,rank-abundance distribution curves,heatmaps,rarefaction curve,Venn diagrams.Results:In the model group,the deferribacteres(12.67%)was significantly increased and the flora structure was abnormal.The relative abundance of the main phyla of firmicutes(49.03%)and the bacteroidetes(43.15%)in the prevention group was close to the control group(44.75%,42.08%).Conclusion:Streptococcus lactis L16 prevents intestinal infection of Staphylococcus aureus in mice and regulates the structure of intestinal flora.