基于耐消化肽的核桃主要过敏原Jug r 2的线性表位筛选

由于核桃Jug r 2是核桃中的主要过敏原,Jug r 2的线性表位是引发核桃过敏反应的关键因素,因此筛选Jug r 2线性表位工作是必要的.本研究利用免疫信息学中的DNAStar Protean系统和ABCpred在线工具预测Jug r 2中的B细胞线性表位.利用体外模拟胃肠消化和HPLC-MS/MS对抗消化肽段的一...

Linear Epitopes Screening Study of Major Walnut Allergen Jug r 2 Based on Anti-digestion Peptides

Jug r 2 is the major allergen of walnut.And the linear epitopes on Jug r 2 is a key factor in triggering a severe allergic reaction.Hence,the research on the efficient strategy for analyzing the linear epitopes of Jug r 2 is necessary.In our study,two immunoinformatic tools including DNAStar Protean and ABCpred were used to predict the B cells linear epitopes on Jug r 2.In vitro simulated gastrointestinal digestion and HPLC-MS/MS were used to identify anti-digestion peptides.And anti-digestion peptides were located on the 3D model of Jug r 2.The results showed that eight predicted B cells linear epitopes were AA186-199,AA226-230,AA257-263,AA284-289,AA373-382,AA389-398,AA408-414,AA472-487;eleven anti-digestion peptides were AA215~220,AA250~260,AA323~337,AA351~356,AA363~388,AA428~438,AA470~483,AA488~513,AA514~526,AA527~541,AA545~572.Of all anti-digestion peptides,four coincided with the predicted or true B cells linear epitope sequences and eight coincided with true T cells epitopes sequences.Anti-digestion peptides showed good antigenic properties in hydrophilic and flexible on the localization of Jug r 23D structure.Therefore,the anti-digestion property of food allergens may be a promising direction for studying linear epitopes of Jug r 2.