超声波协同马克思克鲁维酵母Z17胞内粗酶处理降低乳清蛋白的致敏性

以超声预处理过的乳清蛋白为酶解底物,采用OPA法、ELISA分析等手段,探究马克思克鲁维酵母Z17粗酶水解乳清蛋白、降低乳清蛋白致敏性【以α-乳白蛋白(α-LA)和β-乳球蛋白(β-LG)为抗原性表征】的最优超声预处理-酶解条件。结果表明:乳清蛋白水解度受初始pH值和酶解温度的影响显著,α-LA、β-LG抗原性受初始pH值的影响显著,超声间歇时间和超声功率的交互作用对α-LA、β-LG抗原性影响显著。采用响应面法获得马克思克鲁维酵母Z17转化乳清蛋白的最优酶解条件是:超声间歇时间16 s,超声功率400 W,初始pH 6.16,酶解温度18.48℃,预测α-LA抗原性、β-LG抗原性的降低率达到最大值,分别为65.56%和57.96%。

Reduction of Whey Protein Antigenicity by Intracellular Crude Enzyme Treatment of Kluyveromyces marxianus Z17 Combined with Ultrasound Treatment

In this study,the pre-treated whey protein by ultrasound was used as the substrate for enzymatic hydrolysis.OPA and ELISA method were used to analyze the optimal conditions of ultrasonic pretreatment-enzymatic hydrolysis for whey protein by the crude enzyme of Kluyveromyces marxianus Z17,in order to reduce the antigenicity of whey protein(characterized by the antigenicity ofα-LA andβ-LG).The results showed that the hydrolysis degree of whey protein was significantly affected by initial pH value and hydrolysis temperature,and the antigenicity ofα-LA andβ-LG was apparently influenced by initial pH value,and the interaction of ultrasonic interval time and ultrasonic power also played a role in affecting the antigenicity ofα-LA andβ-LG.The optimal conditions for enzymatic hydrolysis to transform whey protein by Kluyveromyces marxianus Z17 were obtained by response surface method:the reduction rates ofα-LA antigenicity andβ-LG antigenicity were predicted to reach the maximum at the ultrasonic interval time of 16 s,the ultrasonic power of 400 W,the initial pH of 6.16 and the enzymatic hydrolysis temperature of 18.48℃,which were 65.56%and 57.96%,respectively.