柱前衍生高效液相色谱法测定乳制品中甘氨酸的含量

采用单磺酰氯柱前衍生高效液相色谱法测定乳制品中甘氨酸的含量.应用单磺酰氯作为柱前衍生试剂,55℃温度下,反应15min.衍生产物用C18柱(3.9mm×150mm,5μm)分离,0.1mol/L醋酸钠(pH7.2):乙腈75 : 25为流动相进行洗脱,流速1.0mL/min,在330nm的波长下,能准确检测出乳制品中游离甘氨酸.方法回收率在98.2%102%之间,在0205mg/L范围内,线性方程相关系数为0.99994,相对标准偏差RSD为0.87%.该方法衍生反应简单,时间短.衍生产物在330nm具有较强的紫外吸收,且产物单一稳定,冰箱中存放可稳定一星期以上,分析速度快,7min内可与其他峰完全分离.本方法具有操作简单﹑结果重复性好﹑准确等优点.

Determination of glycin in dairy products by pre-column derivatization HPLC

The glycin in dairy products was determined by dansyl chloride as the pre-column derivation reagent at 55℃ for 15min.The derivation can be detected easily and accurately by HPLC.The pre-column derivation was separated by C18 column(3.9 mm × 150mm,5μm),and eluted by mobile phase of 0.1mol/L sodium acetate(pH7.2)and acetonitrile with proportion of 75 : 25,detected at 330nm wavelength.In 7minutes,the derivation was effectively separated.The recovery was between 98.2%102%,the correlation was 0.99994 with the range between 0205mg/L.The derivation was a single product and had strong UV absorption at 330nm.It was stable for more than one week in the refrigerator.The method had the advantage of simple operation,good repetition and accuracy.