秦皮不同溶剂提取物总酚含量及抗氧化活性

分别以水、加0.5%纤维素酶水和50%乙醇溶液为提取剂,超声辅助提取秦皮,采用Folin-Ci-ocalteu法测定提取物中总酚含量,同时利用清除DPPH.和羟自由基(.OH)法评价秦皮提取物的抗氧化活性。结果表明,不同溶剂水、50%乙醇和加0.5%纤维素酶水的秦皮提取物中总酚含量分别41.68mg/g、49.32mg/g和51.51mg/g;对DPPH.的半清除率(IC50)分别为0.29mL、0.22mL和0.17mL;对.OH的半清除率(IC50)分别为2.4mL、2.0mL和1.3mL。秦皮不同溶剂提取物都具有较强的抗氧化活性,其大小顺序为:加酶水提物>醇提物>水提物,抗氧化活性与提取物中总酚含量成正相关。

Total phenol and antioxidant activities of cortex fraxini extracts by different extracting solvents

In this paper,ultrasound-assisted extraction was used to extract activated components from cortex fraxini using water,0.5% cellulase solution and 50% ethanol as extracting agents.The total contents of phenol from the extracts was determined by Folin-Ciocalteu method and antioxidant activities of cortex fraxini extracts was evaluated by cleaning the DPPH and OH.The results indicate that the total contents of phenol in the extracts were 41.68mg/g、49.32mg/g and 51.51mg/g in water,0.5% cellulase solution and 50% ethanol respectively;semi-cleaning rates(IC50)of DPPH· were 0.29mL、0.22mL and 0.17mL;semi-cleaning rates(IC50)of ·OH were 2.4mL、2.0 mL and 1.3mL.All extracts from three solvents showed strong antioxidant capacity.The order of effectiveness is:cellulose solution>ethanol>water.There was a positive correlation between the antioxidation ability and the total phenol contents of the cortex fraxini extract.