| 亚临界水提取大豆胚芽中异黄酮及低聚糖的研究 |
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| 引用本文： | 王凤荣,宋秀梅,张博雅,张永忠.亚临界水提取大豆胚芽中异黄酮及低聚糖的研究[J].中国粮油学报,2011,26(11):32-35,41. |
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| 作者姓名： | 王凤荣 宋秀梅 张博雅 张永忠 |
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| 作者单位： | 1. 东北农业大学理学院,哈尔滨,150030
2. 东北农业大学理学院,哈尔滨150030;教育部大豆生物学重点实验室,哈尔滨150030 |
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| 摘 要： | 以大豆胚芽为原料、高压反应釜为反应容器,应用亚临界水为溶剂、采用高纯氮气加压提取大豆胚芽中异黄酮及大豆低聚糖。利用紫外分光光度法测定大豆异黄酮的含量。选取液料比、提取时间、提取温度、压力4个因素进行单因素试验,再进行正交试验优化亚临界水提取大豆异黄酮的条件。结果表明:在液料比25:1,浸提温度120℃,浸提时间40 min,浸提压力1.9 MPa的条件下,大豆异黄酮的得率是1.09%,大豆异黄酮粗提物的产率为6.52%,粗提物纯度是2.60%;大豆低聚糖粗提物产率是35.2%。
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| 关 键 词： | 亚临界水 大豆胚芽 异黄酮 大豆低聚糖 |
Study on Sub- critical Water Extraction of Isoflavones and Soybean Oligosaccharides from Soybean Germ |
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| Wang Fengrong,Song Xiumei,Zhang Boya,Zhang Yongzhong.Study on Sub- critical Water Extraction of Isoflavones and Soybean Oligosaccharides from Soybean Germ[J].Journal of the Chinese Cereals and Oils Association,2011,26(11):32-35,41. |
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| Authors: | Wang Fengrong Song Xiumei Zhang Boya Zhang Yongzhong |
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| Affiliation: | Wang Fengrong1 Song Xiumei1 Zhang Boya1 Zhang Yongzhong1,2 (College of Science,Northeast Agricultural University1,Harbin 150030) (Key Laboratory of Soybean Biology,Ministry of Education2,Harbin 150030) |
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| Abstract: | The isoflavones and soybean oligosaccharides were extracted by high purity nitrogen pressurization from raw material of soybean germ in a high pressure reaction vessel with the solvent of sub-critical water.The content of soybean isoflavones was determined by UV spectrophotometry.Single factor experiment was carried out with four factors of solid / liquid ratio,extraction temperature,extraction duration and pressure.Then,the orthogonal experiment was done to optimize the conditions for sub-critical water ex... |
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| Keywords: | sub-critical water soybean germ isoflavones soybean oligosaccharides |
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