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豆粕中转基因成分检测技术研究
引用本文:励建荣,余春燕.豆粕中转基因成分检测技术研究[J].中国粮油学报,2007,22(1):114-117.
作者姓名:励建荣  余春燕
作者单位:浙江工商大学食品生物与环境工程学院,杭州,310035
基金项目:浙江省食品安全重点实验室研究项目
摘    要:针对豆粕中核酸含量低且被严重破坏,DNA难以提出的问题对CTAB法进行优化,有效地从豆粕中提取到可以用于扩增反应的DNA。同时定性检测出外源调控序列CaMV35S启动子和NOS终止子及目的基因Cp4-EPSPS序列,成功建立了豆粕中转基因成分的检测方法。

关 键 词:豆粕  DNA提取  转基因成分  检测
收稿时间:2006-01-02
修稿时间:2006-01-02

Detection of Transgenic Factors in Soy Meal
Li Jianrong,Yu Chunyan.Detection of Transgenic Factors in Soy Meal[J].Journal of the Chinese Cereals and Oils Association,2007,22(1):114-117.
Authors:Li Jianrong  Yu Chunyan
Affiliation:College of Food Science, Biotechnology and Invironmental Engineering, Safety Key Lab of Zhejiang Province, Hangzhou Zhejiang Gongshang University, 310035
Abstract:As the nucleic acid in soy meal was destroyed and the DNA in soy meal was difficult to be extracted,the conditions of method CTAB were optimized and the DNA in soy meal for enlarge reaction in transgenic factor detection procedure was efficiently extracted.Meanwhile,the sequences of promotor CaMV35S,terminator NOS and target gene of Cp4-EPSPS were qualitatively detected.Thus,a detection method of transgenic factors in soy meal has been established.
Keywords:soy meal  DNA extraction  transgenic factors  detection
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