| 低能离子注入油脂高产菌株黏红酵母(Rhodotorula glutinis)的选育 |
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| 引用本文： | 李市场,刘红霞,朱朝阳,王冬冬.低能离子注入油脂高产菌株黏红酵母(Rhodotorula glutinis)的选育[J].中国粮油学报,2011,26(8):31-35. |
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| 作者姓名： | 李市场 刘红霞 朱朝阳 王冬冬 |
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| 作者单位： | 河南科技大学食品与生物工程学院,洛阳,471003 |
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| 基金项目： | 中国科学院等离子体物理研究所暨安徽省离子束生物工程学重点实验室开放基金(2008B004) |
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| 摘 要： | 本文对油脂高产菌株选育过程中的筛选方法进行研究,采用苏丹黑B染色作为定性分析、酸热耦合超声波作为定量分析高产油脂菌株的筛选方法,通过低能离子注入黏红酵母(Rhodotorula glutinis)进行诱变,筛选油脂高产菌株。结果表明:获得了一株油脂高产菌株D30,产量比出发菌株提高33.05%,传代试验表明了D30的遗传稳定性良好,当发酵96 h,其油脂产量达3.10 g/L。另外,对D30发酵动力学进行了研究,当发酵时间为10 d时,其生物量为47.98 g/L(菌体湿重),油脂产量达到7.81 g/L。
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| 关 键 词： | 黏红酵母 油脂 苏丹黑B染色 索氏抽提法 酸热耦合超声波 |
Screening of Lipid-producing Strain Rhodotorula Glutinis by Low-energy Ion Implantation |
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| Li Shichang,Liu Hongxia,Zhu Chaoyang,Wang Dongdong.Screening of Lipid-producing Strain Rhodotorula Glutinis by Low-energy Ion Implantation[J].Journal of the Chinese Cereals and Oils Association,2011,26(8):31-35. |
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| Authors: | Li Shichang Liu Hongxia Zhu Chaoyang Wang Dongdong |
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| Affiliation: | Li Shichang Liu Hongxia Zhu Chaoyang Wang Dongdong (Food and Bioengineering School,Henan University of Science and Technology,Luoyang 471003) |
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| Abstract: | The paper studied the screening of strain with high-yield oil,and Sudan Black B staining and Acid-heating+Ultrasonic extraction were respectively chosen in qualitative and quantitative analysis methods to screen the strain with high-yield oil.Screening of lipid-producing strain Rhodotorula glutinis by low-energy ion implantation was studied as well.As a result,a high-yield mutant strain D30 was obtained,and it's lipid yield was increased by 33.05% than that of original.And it was found that genetic characte... |
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| Keywords: | Rhodotorula glutinis lipid Sudan Black B staining Soxhlet extraction Acid-heating Ultrasonic extraction |
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