响应面法优化麦麸发酵产植酸酶条件的研究

以纳豆芽孢杆菌JSU-2为供试菌株,麦麸皮为唯一固体发酵基质,运用响应面法对其产植酸酶的条件进行优化.首先用24-1部分因子试验设计对影响植酸酶活性的主要变量进行了评价,发现主要影响因子为麸皮与水的比例和培养时间.然后用中心组合试验设计确定主要变量的最佳水平.最佳产酶发酵条件为:粒径为3 mm、麸皮与水的比例为1:9.6、接种量为3 mL和培养时间为25 h.在最佳产酶条件下进行发酵,得到的植酸酶活性为595 U/g麦麸.

Optimization of Phytase Production by Fermentation of Wheat Bran Using Response Surface Methodology

Response surface methodology was applied for the optimization of phytase production by Bacillus sub-tilis ( natto) JSU -2 in solid - substrate fermentation using wheat bran as sole substrate. In the first step of optimization, with 2~(4-1) fractional factorial design, wheat bran to water ratio and cultivation time were found to be the important factors affecting phytase production. The two major variables were subsequently optimized using the central composite experimental design. Results; The obtained optimal conditions for phytase production are particle size 3 mm, wheat bran to water ratio 1:9.6, inoculum size 3 mL and cultivation time 25 h. Under these optimal conditions, the phytase production from B. subtilis (natto) JSU -2 reaches 595 IU/g wheat bran.