| 不同酶水解菜籽蛋白的水解物的抗氧化活性研究 |
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| 引用本文： | 鞠兴荣,胡蓉,王丹丹,宋寅,严梅荣.不同酶水解菜籽蛋白的水解物的抗氧化活性研究[J].中国粮油学报,2011,26(4):47-51. |
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| 作者姓名： | 鞠兴荣 胡蓉 王丹丹 宋寅 严梅荣 |
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| 作者单位： | 南京财经大学食品科学与工程学院,南京,210003 |
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| 摘 要： | 使用alcalase、protamex、flavourzyme、木瓜蛋白酶、中性蛋白酶和胰蛋白酶共6种蛋白酶水解菜籽蛋白,研究水解物清除DPPH自由基能力、还原能力和抑制亚油酸过氧化活性。结果表明,6种蛋白酶水解菜籽蛋白的水解物都具有抗氧化活性,但水解物的抗氧化活性与水解所用酶的种类和水解时间有关,胰蛋白酶和flavourzyme水解物显示了较强的清除DPPH自由基和还原能力,6种蛋白酶水解物抑制亚油酸过氧化活性高于VE,但略低于BHT。alcalase水解菜籽蛋白时,水解10~45 m in的水解物清除DPPH自由基和还原能力较强,延长水解时间并不能提高其抗氧化活性。
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| 关 键 词： | 菜籽蛋白 酶 水解物 抗氧化活性 |
Study on Antioxidant Activity of Rapeseed Protein Hydrolysates (RPH) Obtained by Hydrolysis with Different Proteases |
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| Ju Xingrong,Hu Rong,Wang Dandan,Song Ying,Yan Meirong.Study on Antioxidant Activity of Rapeseed Protein Hydrolysates (RPH) Obtained by Hydrolysis with Different Proteases[J].Journal of the Chinese Cereals and Oils Association,2011,26(4):47-51. |
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| Authors: | Ju Xingrong Hu Rong Wang Dandan Song Ying Yan Meirong |
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| Affiliation: | Ju Xingrong Hu Rong Wang Dandan Song Ying Yan Meirong(College of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing 210003) |
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| Abstract: | Rapeseed protein was hydrolyzed with alcalase,potamex,flavourzyme,papain,neutrase and trypsin to investigate DPPH radical scavenging activity,reducing power and inhibition of linoleic acid peroxidation of the hydrolysates.Results showed that all RPH obtained by hydrolysis with six proteases had antioxidant activity which was related to the variety of the enzyme used and the time of hydrolysis.RPH from hydrolysis with trypsin and flavourzyme had higher activity of DPPH radical scavenging and reducing power.A... |
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| Keywords: | rapeseed protein enzyme hydrolysate antioxidant activity |
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