谷氨酰胺和甘氨酰-谷氨酰胺对断奶仔猪小肠黏膜的影响

选用21日龄断奶的杜大长仔猪108头,按试验要求分为3组,每组3个重复,每重复12头.分别饲喂含1%谷氨酰胺（Gln）和0.3%的甘氨酰-谷氨酰胺（Gly-Gln）的日粮,试验期14天,在断奶前、断奶后7天和14天分别屠宰取样.试验结果表明：（1）断奶后7天,日粮中添加Gln和Gly-Gln可显著提高小肠各段绒毛的高度,降低隐窝深度,断奶后14天添加Gly-Gln可显著提高十二指肠、回肠绒毛高度.（2）断奶后7天和14天,添加Gln和Gly-Gln均可显著提高空肠黏膜蔗糖酶和麦芽糖酶活性,对乳糖酶活性无显著影响.（3）断奶后7天,添加Gln和Gly-Gln可降低血浆二胺氧化酶（DAO）活性,提高空肠黏膜DAO活性,断奶后14天,添加Gly-Gln可显著提高空肠黏膜DAO活性.

Effects of Glutamine and Glycyl- Glutamine on Intestinal Mucous in Weaned Piglets

One hundred and eight piglets weaned at 21d age were allocated to three treatments,each of which had 3 replicates,12 piglets per replicate.The three treatments were fed basal diet(control group),basal diet +1% glutamine,and basal diet+0.3% glycyl-glutamine respectively.The results show that:(1) Piglets fed glutamine or glycyl-glutamine have significantly higher intestinal villus height and lower crypt depth at 7d post weaning.Piglets fed glycyl-glutamine have significantly higher duodenum and ileum villus height at 14d post weaning.(2) Supplement with glutamine or glycyl-glutamine significantly increases the activities of sucrase and maltase in jejunum mucous at 7d and 14d post weaning,no significant difference for lactase activity.(3) Supplement with glutamine or glycyl-glutamine significantly decreases the activity of diamine oxidase(DAO) in blood and increases the activity of DAO in jejunum at 7d post weaning.Supplement with glycyl-glutamine significantly increases the activity of DAO in jejunum at 14d post weaning.