Scleral lens wear: Measuring inflammation in the fluid reservoir |
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| Affiliation: | 1. Bausch & Lomb Contact Lens Centre, L V Prasad Eye Institute, Road No. 2, Banjara Hills, Hyderabad, 500034, Telangana, India;2. Bausch and Lomb School of Optometry, Brien Holden Institute of Optometry and Vision Sciences, L V Prasad Eye Institute, Road No. 2, Banjara Hills, Hyderabad, 500034, Telangana, India;3. Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L V Prasad Eye Institute, Road No. 2, Banjara Hills, Hyderabad, 500034, Telangana, India;1. Clinical Instructor Université de Montréal, École d’optométrie, Canada;2. Professor- Université de Montréal, École d’optométrie, 3744 Jean-Brillant, Suite 190-70, Montréal H3T 1P1, Canada;1. Clinical Research Center, School of Optometry, University of California, Berkeley, United States;2. Vision Science Graduate Program, University of California, Berkeley, United States;3. Chemical and Biomolecular Engineering Department, University of California, Berkeley, United States;1. Barnard and Levit Optometrists, Zamenhof House, 58 Clifton Gardens, London, NW11 7EL, UK;2. Institute of Optometry, 56-62 Newington Causeway, London, SE1 6DS, UK;3. London South Bank University, School of Health and Social Care, 103 Borough Rd, London, SE1 0AA, UK;4. Ophthalmology Department, Central Middlesex Hospital, Acton Ln, Park Royal, London, NW10 7NS, UK |
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| Abstract: | PurposeTo measure inflammatory mediators in the scleral lens fluid reservoir (FR) in healthy eyes and to compare them to basal tear samples after 8-hs (8h) and 4-days (4d) of scleral lens (SL) wear.MethodsFifteen normal, habitual soft contact lens wearers were fitted with 14.8- or 15.4-mm SLs (Zenlens, Alden Optical, USA). Basal ocular surface tears and FR samples were collected after 8h and 4d of daily SL wear. Levels of interleukin (IL) -4 and -8, matrix metalloproteinase (MMP)-7, -9, and -10, and tissue inhibitor of MMPs (TIMPs) 1–4 were measured in all samples using Luminex assays. Visual acuity, corneal and conjunctival staining, and comfort assessments were completed at the baseline, 8h and 4d time points.ResultsMMP-9 and MMP-10 were greater in FR than basal ocular surface tears. After 8h of SL wear, the median concentration of MMP-9 in the FR and basal tears were 62.7 and 15.2 ng/mL, respectively (p = 0.047). Likewise, MMP-10 was significantly greater in FR compared to basal tears, after 8h (25.8 ng/mL vs 2.8 ng/mL, p < 0.001) and 4d (2.1 ng/mL vs17.2 ng/mL, p = 0.047). IL-4 and IL-8 levels were greater in FR but not significantly at 8h (2.2 vs 3.1 ng/mL; and 0.1 vs 0.4 ng/mL, respectively) or 4d (0.9 vs 3.5 ng/mL; 0.0 vs 0.2 ng/mL). MMP-7 was not affected by SL wear after 8h (46.0 basal vs 54.4 ng/mL FR) or 4d (34.2 vs 87.5 ng/mL). Visual acuity, corneal and conjunctival staining did not change; comfort was reduced in SL compared to soft contact lens wear.ConclusionsThis is the first study to compare the FR with the basal ocular surface tears. MMP-9 and MMP-10 were elevated in the FR after several hours of SL wear, suggesting potential clinical implications of SL wear and deserves further investigation. |
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| Keywords: | MMP-10 Fluid reservoir MMP-9 Scleral lenses |
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