Antiamoebic properties of Methyltrioctylammonium chloride based deep eutectic solvents |
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| Affiliation: | 1. College of Arts and Sciences, American University of Sharjah, University City, Sharjah 26666, United Arab Emirates;2. Department of Chemical Engineering, College of Engineering, American University of Sharjah, P.O. Box 26666, Sharjah, United Arab Emirates;3. Department of Clinical Sciences, College of Medicine, University of Sharjah, University, City, Sharjah 27272, United Arab Emirates;4. Department of Chemistry, University of Science & Technology, Banuu 28100, Khyber Pakhtunkhwa, C;5. Istinye Faculty of Medicine, Istinye University, Istanbul, Turkey.;1. Department of Ophthalmology, King Saud Medical City, Riyadh, Saudi Arabia;2. College of Medicine, Cairo University, Cairo, Egypt;3. Department of Vision Sciences, Glasgow Caledonian University, Glasgow, United Kingdom;4. Department of Optometry, College of Applied Medical Sciences, Qassim University, Saudi Arabia;1. Höhere Fachschule für Augenoptik Köln, Cologne School of Optometry, Cologne, Germany;2. School of Health and Life Sciences, Aston University, Birmingham, United Kingdom;1. ELZA Institute, Dietikon, Switzerland;2. Paulista School of Medicine, Federal University of Sao Paulo, Sao Paulo, Brazil;3. Faculty of Medicine, University of Geneva, Geneva, Switzerland;4. School of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium;5. School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China;6. USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA;7. University of Zurich, CABMM, Laboratory for Ocular Cell Biology Group, Zurich, Switzerland |
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| Abstract: | PurposeThis aim of this study was to assess anti-parasitic properties of deep eutectic solvents against eye pathogen, Acanthamoeba, often associated with the use of contact lens.MethodsAssays were performed to investigate the effects of various Methyltrioctylammonium chloride-based deep eutectic solvents on Acanthamoeba castellanii, comprising amoebicidal assays, encystment assays, excystment assays, cytotoxicity assays by measuring lactate dehydrogenase release from human cells, and cytopathogenicity assays to determine parasite-mediated host cell death.ResultsIn a 2 h incubation period, DES-B, DES-C, DES-D, and DES-E exhibited up to 85 % amoebicidal activity at micromolar doses, which was enhanced further following 24 h incubation. When tested in encystment assays, selected deep eutectic solvents abolished cyst formation and were able to block excystment of A. castellanii. All solvents exhibited minimal human cell cytotoxicity except DES-D. Finally, all tested deep eutectic solvents inhibited amoeba-mediated cytopathogenicity, except DES-B.ConclusionsDeep eutectic solvents show potent antiamoebic effects. These findings are promising and could lead to the development of novel contact lens disinfectants, as well as opening several avenues to explore the molecular mechanisms, various doses and incubation periods, and use of different bases against Acanthamoeba castellanii. |
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| Keywords: | Adhesion Cytotoxicity Cytopathogenicity Encystation Excystation |
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