Growth and Survival Differences of Vibrio vulnificus and Vibrio parahaemolyticus Strains during Cold Storage

ABSTRACT:  Vibrio vulnificus  and  Vibrio parahaemolyticus  are the most common  Vibrio  species associated with seafood illness in the United States. Our study was conducted to determine if strain-to-strain differences exist in the growth and survival of 8 different  V. vulnificus  and  V. parahaemolyticus  strains at low temperatures. By day 10,  V. vulnificus  strain 515-4C2 had significantly higher counts ( P  < 0.05) (1.97 log CFU/g) compared with strains 3315, 1007, 29306 at 5 °C, which reached nondetectable levels. At 8 °C, strain 515-4C2 had significantly higher counts ( P  < 0.05) (2.23 log CFU/mL) compared with 1007, 33815, 541(O) 49C, which reached nondetectable levels. At 10 °C, only  V. vulnificus  strain 33815 reached nondetectable levels. At 5 °C,  V. parahaemolyticus  strain 541(O) 57C had the highest counts (5.28 log CFU/g) by day 10 while strain 33847 had significantly lower counts (3.46 log CFU/g). After 10 d at 8 °C,  V. parahaemolyticus  strain M350A had the highest counts (7.97 log CFU/mL) while strain 541(O) 57C had the lowest counts (4.80 log CFU/mL). At 10 °C,  V. parahaemolyticus  strain NY477 had significantly higher counts ( P  < 0.05) with 8.31 log CFU/mL compared with strain 33847, which had the lowest counts (6.77 log CFU/mL). Our research has shown that various  V. vulnificus  and  V. parahaemolyticus  strains vary in their ability to survive and grow at refrigeration temperatures.