| 绿色木霉液体发酵产纤维素酶的培养基优化 |
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| 引用本文： | 董玲玲,李志军,田毅红,曾晶,邹坤,龚大春.绿色木霉液体发酵产纤维素酶的培养基优化[J].酿酒科技,2010(2):27-30. |
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| 作者姓名： | 董玲玲 李志军 田毅红 曾晶 邹坤 龚大春 |
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| 作者单位： | 1. 三峡大学-艾伦麦克德尔米德再生能源实验室,湖北,宜昌,443002
2. 安琪酵母股份有限公司,湖北,宜昌,443002 |
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| 基金项目： | 湖北省教育厅高校产学研合作资助项目(CXY2009B2008);;横向项目“纤维素乙醇的相关关键技术的研究”(200800230) |
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| 摘 要： | 研究液体摇瓶发酵产纤维素酶的最佳培养基组成。通过单因素实验确定了培养基中氮源、碳源和诱导碳源的种类，采用正交试验确定了培养基中4种主要营养成分的组成。结果表明，摇瓶液体发酵产纤维素酶的最佳产酶培养基的种类和组成为：有机氮源蛋白胨和无机氮源硫酸铵的含量分别为11g／L和13g／L，葡萄糖6g/L，磷酸二氢钾10．5g/L，爆破的稻草为13．4g/L；纤维素酶的滤纸酶活为19．22U／mL。
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| 关 键 词： | 微生物 纤维素酶 绿色木霉 液体发酵 培养基优化 |
Optimization of Culture Medium for Cellulase Production by Liquid Fermentation of Trihoderma viride |
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| DONG Ling-ling,LI Zhi-jun,TIAN Yi-hong,ZENG Jing,ZOU Kun,GONG Da-chun.Optimization of Culture Medium for Cellulase Production by Liquid Fermentation of Trihoderma viride[J].Liquor-making Science & Technology,2010(2):27-30. |
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| Authors: | DONG Ling-ling LI Zhi-jun TIAN Yi-hong ZENG Jing ZOU Kun GONG Da-chun |
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| Affiliation: | 1.Alan G.Macdiamid Institute of Renewable Energy/a>;Three Gorges University/a>;Yichang/a>;Hubei 443002/a>;2.Angel Yeast Co.Ltd./a>;China |
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| Abstract: | The best culture medium for the preparation of cellulase by liquid fermentation of Trihoderma viride was determined in this paper.Firstly,the species of nitrogen source,carbon source and induced carbon source were determined through single factor test.Then four main compositions of culture medium were determined through orthogonal experiments.The results showed that the compositions of the best culture medium for cellulase preparation were as follows: peptone content(organic nitrogen source) was 11 g/L,(NH4... |
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| Keywords: | microbe cellulase Trichoderma viride liquid fermentation culture medium optimization |
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