Lactococcus lactis subsp. cremoris strain JFR1 attenuates Salmonella adhesion to human intestinal cells in vitro |
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| Affiliation: | 1. Canadian Research Institute for Food Safety, University of Guelph, Guelph, ON N1G 2W1, Canada;2. Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada;3. Dairy Science Department, South Dakota State University, Brookings, SD 57007, USA;1. MRC-Arthritis Research UK Centre for Integrated research into Musculoskeletal Ageing (CIMA), Department of Oncology and Metabolism, University of Sheffield, UK;2. Mellanby Centre for Bone Research, Department of Oncology and Metabolism, University of Sheffield, UK;3. Institute for Cellular Medicine, Newcastle University, UK |
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| Abstract: | Lactococcus lactis subsp. cremoris JFR1 has been studied in reduced fat cheese due to its ability to produce exopolysaccharides (EPS) in situ, contributing to improved textural and organoleptic properties. In this study, the effect of strain JFR1 on virulence gene expression and attachment of Salmonella to HT-29 human colon carcinoma cells was investigated. Overnight cultures of L. lactis subsp. cremoris JFR1 containing EPS, grown in M17 media with 0.5% glucose supplementation, decreased attachment as well as down regulated virulence gene expression in Salmonella enterica subsp. enterica when tested on HT-29 cells. However, EPS isolated from milk fermented with L. lactis subsp. cremoris JFR1 did not affect Salmonella virulence gene expression or attachment to HT-29 cells. These results suggest that EPS does not contribute to the attachment of Salmonella to human intestinal cells. However, the possibility that the isolation process may have affected the structural features of EPS cannot be ruled out. |
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