| 头孢菌素C发酵过程中豆油酶解条件的优化 |
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| 引用本文： | 任强,赵林,刘新利.头孢菌素C发酵过程中豆油酶解条件的优化[J].食品与药品,2014(4):250-253. |
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| 作者姓名： | 任强 赵林 刘新利 |
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| 作者单位： | 齐鲁工业大学山东省微生物重点实验室,山东济南250353 |
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| 基金项目： | 省级博士后创新基金会（201203006）;山东省科技支撑计划项目（2012GSF12107） |
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| 摘 要： | 目的提高头孢菌素C的产量。方法利用响应面法优化豆油的酶解条件,检测酶解后豆油对头孢菌素C产量的影响。结果豆油的最佳酶解条件为水油比0.93,酶解温度43.32℃,酶量0.18%。豆油的酶解率达最高83.29%。以此条件下酶解的豆油为碳源时,头孢菌素C的最终产量提高了51%。结论以酶解后的豆油为碳源时,头孢菌素C的产量明显提高。
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| 关 键 词： | 豆油 酶解 响应面 头孢菌素C |
Optimization of Soybean Off Enzymolysis Condition during Fermentation of Cephalosporin C |
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| REN Qiang,ZHAO Lin,LIU Xin-li.Optimization of Soybean Off Enzymolysis Condition during Fermentation of Cephalosporin C[J].Food and Drug,2014(4):250-253. |
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| Authors: | REN Qiang ZHAO Lin LIU Xin-li |
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| Affiliation: | (Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology, Jinan 250353, China) |
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| Abstract: | Objective To improve the production of cephalosporin C. Methods Response surface method was used to optimize the enzymolysis condition of soybean oil, which was used during the fermentation of cephalosporin C after enzymolysis. The influence of enzymatic hydrolysate of soybean oil on production of cephalosporin C was also determined. Results The optimal reaction conditions were found to be： ratio of water to oil of 0.93, enzymolysis temperature of 43.32 ~C and lipase amount of 0.l 8 %. The enzyme-degradation rate of soybean oil reached the highest 83.29 %. The addition of enzymatic hydrolysate of soybean oil increased cephalosporin production by 51%. Conclusion The enzymatic hydrolysate of soybean oil as carbon source can enhance the production of cephalosporin C significantly. |
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| Keywords: | soybean oil enzymolysis response surface cephalosporin C |
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