莱菔子中芥子碱硫氰酸盐在人工胃肠液中的稳定性研究

目的考察莱菔子水提液中芥子碱硫氰酸盐在人工胃肠液中的稳定性。方法采用高效液相色谱法测定莱菔子水提液中芥子碱硫氰酸盐在未加胃蛋白酶人工胃液、人工胃液和未加胰酶人工肠液、人工肠液中不同时间的峰面积,计算各自质量浓度及平均剩余百分率。结果芥子碱硫氰酸盐在人工胃液中比在人工肠液中稳定,在未加胰酶人工肠液中最不稳定;其次芥子碱硫氰酸盐在两种人工胃液中稳定性相差较小,在两种人工肠液中相差较大。结论芥子碱硫氰酸盐在人工胃肠液中剩余百分率均大于70%,性质较稳定,为其吸收机制提供了参考。

Stability of Sinapine Thiocyanate from Water Extract of Semen Raphani in Artificial Gastric and Intestinal Juice

Objective To investigate the stability of sinapine thiocyanate in artificial gastric and intestinal juice gained from the water extract of Semen Raphani.Methods HPLC was used to determine the area at different time points of sinapine thiocyanate in artificial gastric juice with or without pepsin and artificial intestinal juice with or without pancreatin,the mass concentration and average remaining percentage were calculated respectively.Results Sinapine thiocyanate was more stable in artificial gastric juice than in artificial intestinal juice and most unstable in artificial intestinal juice without pancreatin.The stability of sinapine thiocyanate was similar in two kinds of artificial gastric juice but considerable different in artificial intestinal juice.Conclusion The remaining percentage of sinapine thiocyanate in artificial gastric and intestinal juice,which were relatively stable,were all above 70 ％,and that provided reference for the absorption mechanism of sinapine thiocyanate.