| 酶解牛乳铁蛋白制备乳铁素 B的酶解条件研究 |
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| 引用本文： | 张东送,庞广昌,胡志和,王彦,刘萍.酶解牛乳铁蛋白制备乳铁素 B的酶解条件研究[J].中国乳品工业,2004,32(5):13-15. |
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| 作者姓名： | 张东送 庞广昌 胡志和 王彦 刘萍 |
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| 作者单位： | 1. 天津商学院,生物工程系,天津,300134
2. 海军后勤学院,天津,300450 |
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| 基金项目： | 国家自然科学基金资助项目(30170685) |
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| 摘 要： | 乳铁素B是牛乳铁蛋白经胃蛋白酶酶解后所得到的最重要的抗菌肽，乳铁蛋白酶解生成乳铁素后抗菌活性大大增强。通过正交试验确立了牛乳铁蛋白酶解制备乳铁素B的最佳条件，指出在底物质量浓度为50g／L，酶与底物质量浓度比为1：30(质量比)，酶解时间为60min时所得的乳铁素质量浓度及活性较好。
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| 关 键 词： | 乳铁素B 牛乳 铁蛋白 酶解条件 抗菌活性 |
| 文章编号： | 1001-2230(2004)05-0013-03 |
Study on the conditions of producing lactoferricin B by pepsin digestion of bovine lactoferrin |
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| ZHANG Dong song,PANG Guang chang,HU Zhi he,WANG Yan,LIU Ping.Study on the conditions of producing lactoferricin B by pepsin digestion of bovine lactoferrin[J].China Dairy Industry,2004,32(5):13-15. |
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| Authors: | ZHANG Dong song PANG Guang chang HU Zhi he WANG Yan LIU Ping |
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| Affiliation: | ZHANG Dong song1,PANG Guang chang1,HU Zhi he1,WANG Yan2,LIU Ping2 |
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| Abstract: | Lactoferricin B (Lfcin B) is the main antimicrobial peptide generated by pepsin digestion of bovine lactoferrin(Lf). Lfcin B also shows a significant antibacterial activity than that of Lf. This paper try to get the optimum enzyme hydrolysis conditions by orthogonal experiments. The results indicated.that the optimum enzyme hydrolysis conditions were substrate 5%, enzyme:substrate ratio 1:30 and time 60 minutes |
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| Keywords: | lactoferrin lactoferricin enzyme hydrolysis antibacterial activity |
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