| 耐热型复合乳酸菌冻干保护剂的研究 |
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| 引用本文： | 孙盈,黄金海,李莹,贾程坤,高嵽,刘阳,鲁珂成.耐热型复合乳酸菌冻干保护剂的研究[J].中国乳品工业,2011,39(4). |
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| 作者姓名： | 孙盈 黄金海 李莹 贾程坤 高嵽 刘阳 鲁珂成 |
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| 作者单位： | 天津大学化工学院,天津,300072 |
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| 基金项目： | 天津市应用基础及前沿技术研究计划项目(08JCZDJC22600) |
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| 摘 要： | 为获得对于混合乳酸杆菌最佳的耐热冻干保护剂配方,考察了不同冻干剂单因子添加物及5种冻干保护剂配方对冷冻干燥后乳酸菌的存活率、耐热性能,失水情况和感官指标,利用中心组合响应曲面法设计,获得以活菌率,耐热性和失水率为控制指标的保护剂组分优化的多元二次方程,并进行显著性分析。结果表明,通过初步筛选获得感官良好、复溶后相容性好的冻干介质,进一步条件优化得到最佳配方。最佳冻干保护剂配方为脱脂乳10%,聚乙烯吡咯烷酮(PVP)5%,海藻糖5%,维生素C为0.5%(均为质量分数),此时冻干后活菌率的预测值可达到93.05%,37℃保存10 d后活菌率预测值可达到77.26%,具有较好的耐热性能。
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| 关 键 词： | 乳酸菌 冻干保护剂 中心组合设计 耐热性 |
Study on freeze-drying protectant formula with heat-tolerance of complex Lactic acid bacteria |
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| SUN Ying,HUANG Jin-hai,LI Ying,JIA Cheng-kun,GAO Die,LIU Yang,LU Ke-cheng.Study on freeze-drying protectant formula with heat-tolerance of complex Lactic acid bacteria[J].China Dairy Industry,2011,39(4). |
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| Authors: | SUN Ying HUANG Jin-hai LI Ying JIA Cheng-kun GAO Die LIU Yang LU Ke-cheng |
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| Affiliation: | SUN Ying,HUANG Jin-hai,LI Ying,JIA Cheng-kun,GAO Die,LIU Yang,LU Ke-cheng(School of Chemical Engineering,Tianjin University,Tianjin 300072,China) |
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| Abstract: | Aim: Effect of different additions was to be analysed and optimal freeze-drying protectant formula with heat-tolerance of comolex Lactic Acid Bacteria strains(LABs) was to be obtained.Method: Detection of bacterial livability,heat-tolerance,driage and oranoleptic indicator among five freeze-drying protectant formulas and different additives was performed.Then the central composite response surface was employed to obtain the multiple quadratic equations on bacterial livability,heat-tolerance and driage and s... |
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| Keywords: | lactic acid bacteria freeze-drying protectant central composite heat-tolerance |
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