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利用16S rDNA序列及tuf-RFLP鉴定蒙古国发酵乳中的乳酸菌
引用本文:王宏梅,于洁,包秋华,吕嫱,孟和毕力格,张和平.利用16S rDNA序列及tuf-RFLP鉴定蒙古国发酵乳中的乳酸菌[J].中国乳品工业,2011,39(7).
作者姓名:王宏梅  于洁  包秋华  吕嫱  孟和毕力格  张和平
作者单位:内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特,010018
基金项目:973计划前期研究专项(2010CB134502); 教育部创新团队发展计划(IRT0967); 863计划项目(2011AA100902)
摘    要:运用16S rDNA序列分析和tuf-RFLP技术对采于蒙古国扎布汗省的25份发酵乳样中分离出的110株乳酸菌进行鉴定。首先将分离的110株乳酸菌的16S rRNA基因进行扩增,测序并构建系统发育树,初步鉴定为41株嗜热链球菌,40株瑞士乳杆菌,11株德氏乳杆菌保加利亚亚种,2株发酵乳杆菌,1株乳明串珠菌,2株肠膜明串肠膜亚种,1株乳酸乳球乳酸亚种和12株属于干酪族的菌株。由于干酪乳杆菌族的16S rDNA序列差异很小,故采用tuf-RFLP技术对这12株进行了进一步的验证,通过分离菌株与模式菌株tuf-RFLP图谱的比较分析,结果表明这12株菌均为干酪乳杆菌。

关 键 词:乳酸菌  16S  rDNA序列分析  系统发育树  tuf-RFLP  

Identification Lactic acid bacteria isolated from mongolia fermented milk products bases on 16S rDNA sequence analysis and tuf-RFLP technology
WANG Hong-mei,YU Jie,BAO Qiu-hua,LV Qiang,Menghebelige,ZHANG He-ping.Identification Lactic acid bacteria isolated from mongolia fermented milk products bases on 16S rDNA sequence analysis and tuf-RFLP technology[J].China Dairy Industry,2011,39(7).
Authors:WANG Hong-mei  YU Jie  BAO Qiu-hua  LV Qiang  Menghebelige  ZHANG He-ping
Affiliation:WANG Hong-mei,YU Jie,BAO Qiu-hua,LV Qiang,Menghebelige,ZHANG He-ping(Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010018,China)
Abstract:One hundred and ten strains of lactic acid bacteria(LAB) isolated from twenty five samples of naturally fermented yoghurt in Dzavhan of Mongolia were classified by 16S rDNA sequence analysis and tuf-RFLP.They were preliminary identified as Streptococcus thermophilus(41 strains),Lactobacillus helveticus(40 strains),Lactobacillus delbrueckii subsp.bulgaricus(11 strains),Lactobacillus fermentum(2 strains),Leuconostoc mesenteroides subsp.mesenteroides(2 strains),Lactococcus lactis subsp.lactis(1 strain) and Lac...
Keywords:Lactic acid bacteria  16S rDNA sequence analysis  phylogenetic tree  tuf-RFLP  
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