| 光照调整对于牛乳中褪黑素质量浓度的影响及其检测 |
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| 引用本文： | 任皓威,王丹,刘鹏,姜毓君.光照调整对于牛乳中褪黑素质量浓度的影响及其检测[J].中国乳品工业,2011,39(4). |
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| 作者姓名： | 任皓威 王丹 刘鹏 姜毓君 |
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| 作者单位： | 东北农业大学黑龙江省乳品工业技术开发中心,哈尔滨,150086 |
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| 基金项目： | 黑龙江省优势农产品生产与加工研发平台产业化项目(193082) |
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| 摘 要： | 通过调整奶牛的光照周期使牛乳中富含褪黑素成分,并通过液相色谱技术对于富含褪黑素的原料乳进行检测,结果发现褪黑素质量浓度有显著提高。色谱条件:色谱柱Zorbax eclipse XDB C18柱;流动相为甲醇∶水=60∶40,流速为1 mL/min,荧光检测器Ex=286 nm,Em=345 nm。
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| 关 键 词： | 褪黑素 光照 牛乳及乳制品 检测 |
Influence of light rhythm on melatonin in milk and detection |
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| REN Hao-wei,WAN Dan,LIU Peng,JIANG Yu-jun.Influence of light rhythm on melatonin in milk and detection[J].China Dairy Industry,2011,39(4). |
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| Authors: | REN Hao-wei WAN Dan LIU Peng JIANG Yu-jun |
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| Affiliation: | REN Hao-wei,WAN Dan,LIU Peng,JIANG Yu-jun(Heilongjiang Dairy Industry Technical Development Center,Northeast Agricultural University,Harbin 150086,China) |
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| Abstract: | In order to get the melatonin rich milk,the method by adjusting the light rhythm of cows was used.And a method for determination of melatonin in milk and dairy products by high-performance liquid chromatography(HPLC) was used.And then separated on Zorbax eclipse XDB C18 column with a mixed solution of methanol/water 60/40 as mobile phase,and detected at Ex=286 nm,Em=345 nm with a Fluorescence detector. |
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| Keywords: | Melatonin light milk and dairy products detection |
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