| 酪蛋白类蛋白物的溶剂分级和酶水解对ACE抑制活性的影响 |
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| 引用本文： | 孙辉,赵新淮.酪蛋白类蛋白物的溶剂分级和酶水解对ACE抑制活性的影响[J].中国乳品工业,2011,39(12). |
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| 作者姓名： | 孙辉 赵新淮 |
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| 作者单位： | 东北农业大学乳品科学教育部重点实验室,哈尔滨,150030 |
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| 基金项目： | 黑龙江省高等学校科技创新团队建设计划项目(2010td11) |
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| 摘 要： | 利用响应面法优化类蛋白反应条件修饰酪蛋白水解物制备酪蛋白类蛋白物.酪蛋白类蛋白物的ACE抑制活性高于酪蛋白水解物,IC50值从52.6 mg/L降低到14.9 mg/L.利用乙醇-水混合溶剂对酪蛋白水解物和类蛋白物进行分级,结果表明,极性最低的溶剂得到的上清液部分活性较高,而沉淀部分活性较低.4种蛋白酶水解酪蛋白类蛋白物的分级产物,导致活性下降,除碱性蛋白酶外,木瓜蛋白酶、胃蛋白酶和胰蛋白酶的水解产物ACE抑制活性为31.5％～46.8％,但是仍然高于酪蛋白水解物的ACE抑制活性(27.8％),表明类蛋白反应提高了酪蛋白水解物对一些蛋白酶的体外抵抗能力.
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| 关 键 词： | 酪蛋白水解物 类蛋白反应 碱性蛋白酶 乙醇分级 ACE抑制活性 |
Impact of solvent fractionation and enzymatic hydrolysis of casein hydrolysate modified by plastein reaction on its ACE inhibition |
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| SUN Hui
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ZHAO Xin-huai.Impact of solvent fractionation and enzymatic hydrolysis of casein hydrolysate modified by plastein reaction on its ACE inhibition[J].China Dairy Industry,2011,39(12). |
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| Authors: | SUN Hui ZHAO Xin-huai |
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| Affiliation: | SUN Hui,ZHAO Xin-huai(Northeast Agricultural University Key Laboratory of Dairy Science,Ministry of Education,Harbin 150030,China) |
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| Abstract: | The prepared hydrolysate was modified by the plastein reaction under the response-surface-methodology optimized conditions to prepare casein plasteins.The casein plasteins exhibited a higher ACE-inhibitory activity in vitro than the original hydrolysate as its value of IC50 was decreased from original 52.6 to 14.9 g/mL.When the original hydrolysate or the plasteins was fractionated by ethanol-water solvents respectively,the soluble fractions obtained by the solvent of the lowest polarity had a higher activi... |
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| Keywords: | casein hydrolysate plastein reaction alcalase ethanol fractionation ACE-inhibitory activity |
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