| 超滤法浓缩分离免疫初乳中的抗体 |
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| 引用本文： | 张和平,李立民.超滤法浓缩分离免疫初乳中的抗体[J].中国乳品工业,1999,27(4):3-6. |
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| 作者姓名： | 张和平 李立民 |
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| 作者单位： | 东北农业大学畜产品加工研究所，内蒙古农牧学院，东北农业大学畜产品加工研究所 |
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| 摘 要： | 收集经大肠杆菌、沙门氏菌混合疫苗免疫处理的乳牛产后７天之内初乳，经去脂、去酪蛋白后所得免疫初乳乳清，用中空纤维超滤器（分子截留为１００ｋｕ（１００ｋｄ）进行浓缩分离，浓缩４．５～７倍、ＩｇＧ收率在９０％以上。为了制得高纯度ＩｇＧ分离物，可和３５％饱和度的硫胺进行盐析，然后再进行超滤脱盐、浓缩、制得的ＩｇＧ浓缩物纯度达８０％以上，这一工艺适合工业化生产。
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| 关 键 词： | 超滤 免疫初乳 乳抗体 特异性IgG 初乳 抗体 IgG |
Concentration and Seperation of Milk Antibody from Immune Bovine Colostrum by Ultrafiltration |
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| Zhang Heping,Li Limin.Concentration and Seperation of Milk Antibody from Immune Bovine Colostrum by Ultrafiltration[J].China Dairy Industry,1999,27(4):3-6. |
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| Authors: | Zhang Heping Li Limin |
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| Abstract: | The bovine milk antibody against E.Coli and Salmonella was concentrated and seperated from immune colostrum whey using hollow fibre ultrafiltrator (100ku M W cut-off), recovery of IgG is above 90%. In order to obtain higher purity of IgG, precipitation of IgG from whey by adding of (NH 4 ) 2SO 4 up to 35% saturated solution was carried out befor ultrafiltration, the purity of IgG is up to above 80%.This processing technology for IgG concentration and seperation can be scaled up. |
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| Keywords: | ultrafiltration immune colostrum milk antibody(specific IgG) |
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